Establishment and application of an experimental model of human fetal hepatocytes for investigation of the protective effects of silybin and polyporus umbellalus polysaccharides

doi:10.3748/wjg.v3.i4.228

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Dec 15, 1997 (publication date) through Apr 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 15, 1997; 3(4): 228-230
Published online Dec 15, 1997. doi: 10.3748/wjg.v3.i4.228

Establishment and application of an experimental model of human fetal hepatocytes for investigation of the protective effects of silybin and polyporus umbellalus polysaccharides

Mao-Rong Wang, Mei-Zhao Le, Jia-Zhang Xu, Chang-Lun He

Mao-Rong Wang, Mei-Zhao Le, Jia-Zhang Xu, Chang-Lun He, Institute of Liver Diseases, 81^st Hospital, PLA, Nanjing 210002, Jiangsu Province, China

Mao-Rong Wang, male, born on Dec. 17, 1962 in Fanchang, Anhui Province, graduated from the Department of Medicine at Wannan Medical College, currently Physician-in-Charge, engaged in the study of viral hepatitis, having 30 papers published.

Author contributions: All authors contributed equally to the work.

Supported by the Military Scientific Foundation for Youth Scientists, No. 91D049-0300.

Correspondence to: Dr. Mao-Rong Wang, Institute of Liver Diseases, 81^st Hospital, PLA, Nanjing 210002, Jiangsu Province, China

Telephone: +86-25-4419662

Received: August 3, 1996
Revised: August 26, 1996
Accepted: September 13, 1996
Published online: December 15, 1997

Abstract

AIM: To establish a new experimental model system of human fetal hepatocytes to study the mechanisms underlying the protective effect of silybin and polyporus umbellalus polysaccharides (PSP) on the cellular ultrastructure.

METHODS: Human fetal hepatocytes were obtained from the liver of a human fetus that resulted from a medically necessary induced labor; the mother provided informed consent for sampling, experimental use and publication of findings. The hepatocytes were cultured and then pretreated with silybin or PSP or without either (control), after which the treated cells were exposed to CCl₄ for 4 h. Changes in cellular ultrastructure were observed by scanning electron microscopy and transmission electron microscopy, and changes in alanine aminotransferase (ALT), aspartate aminotransferase (AST) and superoxide dismutase (SOD) were assayed.

RESULTS: Levels of ALT and AST were significantly decreased, and level of SOD was elevated in the two pretreatment groups following CCl₄ exposure, as compared to the control group. The cellular integrity and ultrastructure were well preserved in the two pretreatment groups but were seriously damaged in the control group.

CONCLUSION: The CCl₄-induced hepatotoxic cell model system of human fetal hepatocytes is an effective tool for studying the hepatoprotective effect of drugs and may be applicable for studies to screen medicines for treatment of hepatitis.

Keywords: Fetal hepatocytes, Experimental model, Silybin, Polyporus umbellalus polysaccharides