Original Research
Copyright ©The Author(s) 1997. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 1997; 3(3): 153-155
Published online Sep 15, 1997. doi: 10.3748/wjg.v3.i3.153
Effects of Linomide on growth and metastasis of implanted human gastric cancer in nude mice
Hou-Quan Tao, Yan-Zhen Lin, Hao-Ran Yin, Qin-Long Gu, Zheng-Gang Zhu, Ming Yao
Hou-Quan Tao, Yan-Zhen Lin, Hao-Ran Yin, Qin-Long Gu, Zheng-Gang Zhu, Ming Yao, Department of Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China
Hou-Quan Tao, having 9 papers published, Department of Surgery, Ruijin Hospital, Shanghai 200025, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Hou-Quan Tao, Professor, Department of Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China
Telephone: +86-21-64370045
Received: December 24, 1996
Revised: February 19, 1997
Accepted: March 10, 1997
Published online: September 15, 1997
Abstract

AIM: To elucidate the effect of angiogenesis inhibitor, Linomide, on tumor growth and metastasis in nude mice implanted with human gastric cancer.

METHODS: A metastatic model of gastric cancer was established using orthotopic implantation of histologically intact tumor tissues into the gastric wall of nude mice. Linomide (0, 80, 160 mg·kg-1) was given p.o. every day after the implantation, and the mice were sacrificed after 10 wk to detect tumor size and metastasis. The microvessel counts were measured by immunohistochemical staining using a monoclonal antibody against Human Factor VIII related antigen.

RESULTS: Linomide treatment significantly decreased the size of the implanted tumors (control group: 1.36 ± 0.81 cm3vs Linomide treated group: 0.84 ± 0.51 cm3 and 0.62 ± 0.35 cm3, P < 0.05 and 0.01, respectively). Additionally, an antimetastatic effect of Linomide was clearly demonstrated in a dose dependent manner: mice given 80 mg·kg-1 Linomide developed liver metastasis in 4 of 10 cases, mice given 160 mg/kg developed metastasis in only 1 of 10 mice, while it developed in 19 of 28 mice of the control group (P < 0.05 and 0.01, respectively). The number of metastatic foci was also significantly less in the treated group. Furthermore, the microvessel counts in tumors of treated mice was reduced by 33%-42% as compared with the control tumors (P < 0.01).

CONCLUSION: Linomide has a strong inhibitory activity against in vivo tumor growth and metastasis of gastric cancer, effectively suppressing the growth of the primary tumor, preventing liver metastasis, and attenuating the rate of neovascularization.

Keywords: Linomide; Stomach neoplasms; Neoplasm metastasis; Liver neoplasms/secondary; Neovascularization, pathologic