Published online Nov 28, 2018. doi: 10.3748/wjg.v24.i44.5005
Peer-review started: September 11, 2018
First decision: October 11, 2018
Revised: October 20, 2018
Accepted: November 2, 2018
Article in press: November 2, 2018
Published online: November 28, 2018
To develop a novel rat model of heterogeneous hepatic injury.
Seventy male Sprague-Dawley rats were randomly divided into a control group (n = 10) and a colchicine group (n = 60). A 0.25% colchicine solution (0.4 mL/kg) was injected via the splenic vein in the colchicine group to develop a rat model of heterogeneous hepatic injury. An equal volume of normal saline was injected via the splenic vein in the control group. At days 3, 7, and 14 and weeks 4, 8, and 12 after the operation, at least seven rats of the colchicine group were selected randomly for magnetic resonance imaging (MRI) examinations, and then they were euthanized. Ten rats of the control group underwent MRI examinations at the same time points, and then were euthanized at week 12. T2-weighted images (T2WI) and diffusion weighted imaging (DWI) were used to evaluate the heterogeneous hepatic injury. The heterogeneous injury between the left and right hepatic lobes was assessed on liver sections according to the histological scoring criteria, and correlated with the results of MRI study.
Obvious pathological changes occurred in the hepatic parenchyma in the colchicine group. Hepatic injury scores were significantly different between the left and right lobes at each time point (P < 0.05). There was a significant difference in apparent diffusion coefficient (ADC) of DWI and liver-to-muscle ratio (LMR) of T2WI between the left and right lobes of rats in the colchicine group (P < 0.05) at each time point, and similar results were observed between the colchicine and control groups. Besides, there was a significant correlation between hepatic injury scores and ADC values or LMR (r = -0.682, P = 0.000; r = -0.245, P = 0.018).
Injection with colchicine via the splenic vein can be used to successfully develop a rat model of heterogeneous hepatic injury. DWI and T2WI may help evaluate the heterogeneous injury among liver lobes.
Core tip: In this study, injection with colchicine via the splenic vein is shown to successfully develop a rat model of heterogeneous hepatic injury. Obvious pathological changes occurred in the hepatic parenchyma in the colchicine group. A significant difference was observed in liver injury scores, apparent diffusion coefficient values, and liver-to-muscle ratios of T2-weighted images (T2WI) between the left and right lobes in the colchicine group (P < 0.05). Our study suggested that diffusion weighted imaging and T2WI can be used to evaluate the heterogeneous injury among liver lobes.