Published online Dec 7, 2017. doi: 10.3748/wjg.v23.i45.7989
Peer-review started: July 24, 2017
First decision: August 30, 2017
Revised: October 6, 2017
Accepted: October 17, 2017
Article in press: October 17, 2017
Published online: December 7, 2017
To investigate a safer way to set up the disease model of cystic echinococcosis without contamination risk and develop a novel experimental murine model of hepatic cystic echinococcosis.
C57B/6 mice were injected with human protoscolices of three different concentrations via the portal vein. The mice were followed for 10 mo by ultrasound, gross anatomy, and pathological and immunological examinations. The protoscolex migration in the portal vein, hydatid cyst growth, host immune reaction, and hepatic histopathology were examined periodically.
The infection rates in the mice in the high, medium, and low concentration groups were 90%, 100%, and 63.6%, respectively. The protoscolices migrated in the portal vein with blood flow, settled in the liver, and developed into orthotopic hepatic hydatid cysts, resembling the natural infection route and course.
We have established an improved experimental model of hepatic cystic echinococcosis with low biohazard risk but stable growing dynamics and immune reaction. It is especially useful for new anti-parasite medication trials against hydatid disease.
Core tip: In this experimental study, we developed a novel murine model of cystic echinococcosis. This orthotopic model resembles primary infection route and natural infectious course with low biohazard risk and high efficiency.