Observational Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 21, 2017; 23(39): 7168-7173
Published online Oct 21, 2017. doi: 10.3748/wjg.v23.i39.7168
Expression of annexin A5 in serum and tumor tissue of patients with colon cancer and its clinical significance
Chong-Bing Sun, Ai-Yan Zhao, Shuai Ji, Xiao-Qing Han, Zuo-Cheng Sun, Meng-Chun Wang, Fu-Chang Zheng
Chong-Bing Sun, Ai-Yan Zhao, Xiao-Qing Han, Zuo-Cheng Sun, Meng-Chun Wang, Fu-Chang Zheng, Department of General Surgery, Weifang People’s Hospital, Weifang 261000, Shandong Province, China
Shuai Ji, Department of Anorectal Surgery, Linqu People’s Hospital, Weifang 261000, Shandong Province, China
Author contributions: All authors contributed to the manuscript.
Institutional review board statement: This study is approved by the Local Hospital Review Board.
Informed consent statement: All cases enrolled have signed the consent statement.
Conflict-of-interest statement: No conflict of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Fu-Chang Zheng, Associate Chief Physician, Department of General Surgery, Weifang People's Hospital, 151 Guangwen Road, Weifang 261000, Shandong Province, China. zhengfc@yeah.net
Telephone: +86-536-8192599
Received: February 27, 2017
Peer-review started: February 27, 2017
First decision: April 10, 2017
Revised: June 20, 2017
Accepted: August 8, 2017
Article in press: August 8, 2017
Published online: October 21, 2017
Abstract
AIM

To investigate the expression of annexin A5 in serum and tumor tissue of patients with colon cancer and to analyze its clinical significance.

METHODS

Ninety-three patients with colon cancer treated at our hospital between February 2013 and March 2016 were included in an observation group, and 40 healthy individuals were included in a control group. Enzyme-linked immunosorbent assay was performed to determine the serum level of annexin A5, while immunohistochemistry was performed to determine the expression of annexin A5 in cancer tissues.

RESULTS

The serum level of annexin A5 was 0.184 ± 0.043 ng/mL in the observation group, which was significantly higher than that in the control group (P < 0.05). Annexin A5 expression was detected in 79.31% of the patients with lymph node metastasis, which was significantly higher than that in patients without lymph node metastasis (P < 0.05). Moreover, annexin A5 expression was detected in 86.96% of the patients with stage III to IV disease, which was significantly higher than that in patients with stage I to II disease (P < 0.05). The serum level of annexin A5 was 0.215 ± 0.044 ng/mL in patients whose tumors were positive for annexin A5 expression, which was significantly higher than that in patients whose tumors were negative for annexin A5 expression (P < 0.05). The serum level of annexin A5 was correlated with annexin A5 expression in colon cancer tissues (r = 0.312, P < 0.05). When a cutoff value of > 0.148 ng/mL for serum level of annexin A5 was used in the diagnosis of colon cancer, the sensitivity was 83.90%, and the specificity was 57.50%.

CONCLUSION

For patients with colon cancer, annexin A5 expression in cancer tissues is related to lymph node metastasis and tumor grade. Serum level of annexin A5 is related to annexin A5 expression in cancer tissues and is of diagnostic relevance.

Keywords: Immunohistochemistry, Annexin A5, Colon cancer, Serum

Core tip: For patients with colon cancer, annexin A5 expression in cancer tissues is related to lymph node metastasis and tumor grade. Serum level of annexin A5 is related to annexin A5 expression in cancer tissues and is of diagnostic relevance.