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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
Protective effect of Bifidobacterium infantis CGMCC313-2 on ovalbumin-induced airway asthma and β-lactoglobulin-induced intestinal food allergy mouse models
Meng-Yun Liu, Zhen-Yu Yang, Wen-Kui Dai, Jian-Qiong Huang, Yin-Hu Li, Juan Zhang, Chuang-Zhao Qiu, Chun Wei, Qian Zhou, Xin Sun, Xin Feng, Dong-Fang Li, He-Ping Wang, Yue-Jie Zheng
Meng-Yun Liu, Jian-Qiong Huang, He-Ping Wang, Yue-Jie Zheng, Department of Respiratory, Shenzhen Children’s Hospital, Shenzhen 518026, China
Zhen-Yu Yang, Wen-Kui Dai, Yin-Hu Li, Chuang-Zhao Qiu, Qian Zhou, Xin Feng, Dong-Fang Li, WeHealthGene Co., Shenzhen 518129, China
Juan Zhang, Chun Wei, Xin Sun, Xijing Hospital, The Fourth Military Medical University, Xi’an 7100032, Shann’xi Province, China
Author contributions: Zheng YJ designed the study; Liu MY, Yang ZY and Li YH interpreted the data and wrote the manuscript; Qiu CZ, Zhou Q and Feng X conducted the bioinformatics analysis; Li DF, Huang JQ, Zhang J, Wang HP, Wei C and Sun X contributed to the study design and animal experiments; Liu MY, Yang ZY and Dai WK contributed to this work equally.
Supported by Basic Science Research Program funded by The Innovation of Science and Technology Commission of Shenzhen Municipality, China, No. JCYJ20120828092009036; Shenzhen Science and Technology Project, No. JCYJ20150403100317067.
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board (IRB) in Shenzhen Children’s Hospital.
Institutional animal care and use committee statement: The experimental procedures involving animals in this study were reviewed and approved by the Animal Welfare and Ethical Committee in The Fourth Military Medical University (approval ID: 20150902).
Conflict-of-interest statement: The authors declare that they have no competing interests.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Yue-Jie Zheng, Director, Department of Respiratory, Shenzhen Children’s Hospital, No. 7019 Yitian Road, Shenzhen 518026, China. shine1990@sina.com
Telephone: +86-755-83936101 Fax: +86-755-83009800
Received: November 25, 2016
Peer-review started: November 28, 2016
First decision: December 28, 2016
Revised: January 16, 2017
Accepted: February 16, 2017
Article in press: February 17, 2017
Published online: March 28, 2017
AIM
To determine whether oral administration of Bifidobacterium infantis CGMCC313-2 (B. infantis CGMCC313-2) inhibits allergen-induced airway inflammation and food allergies in a mouse model.
METHODS
Ovalbumin (OVA)-induced allergic asthma and β-lactoglobulin-induced food allergy mouse models were used in this study. Following oral administration of B. infantis CGMCC313-2 during or after allergen sensitization, histopathologic changes in the lung and intestine were evaluated by hematoxylin and eosin (HE) staining. In the allergic asthma mouse model, we evaluated the proportion of lung-infiltrating inflammatory cells. OVA-specific IgE and IgG1 levels in serum and cytokine levels in bronchoalveolar lavage fluid (BALF) were also assessed. In the food allergy mouse model, the levels of total IgE and cytokines in serum were measured.
RESULTS
Oral administration of B. infantis CGMCC313-2 during or after allergen sensitization suppressed allergic inflammation in lung and intestinal tissues, while the proportion of infiltrating inflammatory cells was significantly decreased in the BALF of allergic asthma mice. Moreover, B. infantis CGMCC313-2 decreased the serum levels of total IgE in food allergy mice, and reductions in IgE and IgG1 were also observed in OVA-induced allergic asthma mice. The expression of interleukin-4 (IL-4) and IL-13 in both serum and BALF was suppressed following the administration of B. infantis CGMCC313-2, while an effect on serum IL-10 levels was not observed.
CONCLUSION
B. infantis CGMCC313-2 inhibits the secretion of allergen-induced IgE, IL-4 and IL-13, and attenuates allergic inflammation.
Core tip:Bifidobacterium infantis CGMCC313-2 significantly decreased the serum concentration of IgE and IgG1 in asthma and food allergy mouse models. The number of infiltrating cells in bronchoalveolar lavage fluid was reduced, and eosinophil infiltration in lungs was relieved by B. infantis CGMCC313-2 in allergic asthma mice. Body weight was regained in food allergy mice, and intestinal inflammation was attenuated by B. infantis CGMCC313-2. Following administration of B. infantis CGMCC313-2, the concentrations of interleukin-4 (IL-4) and IL-13 decreased in both allergic asthma and food allergy mice.
