Copyright
©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
Melatonin modulates adiponectin expression on murine colitis with sleep deprivation
Tae Kyun Kim, Young Sook Park, Haing-Woon Baik, Jin Hyun Jun, Eun Kyung Kim, Jae Woong Sull, Ho Joong Sung, Jin Woo Choi, Sook Hee Chung, Myung Chan Gye, Ju Yeon Lim, Jun Bong Kim, Seong Hwan Kim
Tae Kyun Kim, Young Sook Park, Jun Bong Kim, Seong Hwan Kim, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, Seoul 139-711, South Korea
Haing-Woon Baik, Department of Biochemistry and Molecular Biology, Eulji University School of Medicine, Daejeon 301-070, South Korea
Jin Hyun Jun, Jae Woong Sull, Ho Joong Sung, Jin Woo Choi, Departemnt of Biomedical Laboratory Science, Graduate School of Health Science, Eulji University, Seongnam-si 13135, South Korea
Jin Hyun Jun, Departemnt of Senior Healthcare, BK21 plus Program, Graduate School, Eulji University, Daejeon 34824, South Korea
Eun Kyung Kim, Department of Pathology, Eulji University School of Medicine, Seoul 139-711, South Korea
Sook Hee Chung, Department of Gastroenterology, Ajou University School of Medicine, Suwon 443-749, South Korea
Myung Chan Gye, Ju Yeon Lim, Department of Life Science and Institute of Natural Sciences, Hanyang University, Seoul 04763, South Korea
Author contributions: All the authors contributed to this manuscript.
Supported by 2012 research fund from Eulji University.
Institutional review board statement: The Institutional Review Board of Eulji Hospital at Eulji University College of Medicine approved the study protocol (Approval number: EUIACUC12-11).
Institutional animal care and use committee statement: All procedures in experiments were conducted according to the Animal Care Guidelines of the National Institutes of Health and Korean Academy of Medical Sciences.
Conflict-of-interest statement: There is no conflict of interest in this study.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Young Sook Park, MD, PhD, Department of Gastroenterology, Eulji University School of Medicine, Eulji Hospital, 68 Hangeulbiseok-Ro, Nowon-Gu, Seoul 139-711, South Korea. pys1109@eulji.ac.kr
Telephone: +82-2-970-8207 Fax: +82-2-970-8621
Received: May 5, 2016
Peer-review started: May 6, 2016
First decision: June 20, 2016
Revised: July 11, 2016
Accepted: July 31, 2016
Article in press: July 31, 2016
Published online: September 7, 2016
AIM
To determine adiponectin expression in colonic tissue of murine colitis and systemic cytokine expression after melatonin treatments and sleep deprivation.
METHODS
The following five groups of C57BL/6 mice were used in this study: (1) group I, control; (2) group II, 2% DSS induced colitis for 7 d; (3) group III, 2% DSS induced colitis and melatonin treatment; (4) group IV, 2% DSS induced colitis with sleep deprivation (SD) using specially designed and modified multiple platform water baths; and (5) group V, 2% DSS induced colitis with SD and melatonin treatment. Melatonin (10 mg/kg) or saline was intraperitoneally injected daily to mice for 4 d. The body weight was monitored daily. The degree of colitis was evaluated histologically after sacrificing the mice. Immunohistochemical staining and Western blot analysis was performed using anti-adiponectin antibody. After sampling by intracardiac punctures, levels of serum cytokines were measured by ELISA.
RESULTS
Sleep deprivation in water bath exacerbated DSS induced colitis and worsened weight loss. Melatonin injection not only alleviated the severity of mucosal injury, but also helped survival during stressful condition. The expression level of adiponectin in mucosa was decreased in colitis, with the lowest level observed in colitis combined with sleep deprivation. Melatonin injection significantly (P < 0.05) recovered the expression of adiponectin. The expression levels of IL-6 and IL-17 were increased in the serum of mice with DSS colitis but decreased after melatonin injection.
CONCLUSION
This study suggested that melatonin modulated adiponectin expression in colonic tissue and melatonin and adiponectin synergistically potentiated anti-inflammatory effects on colitis with sleep deprivation.
Core tip: We report this first study that melatonin and sleep deprivation are related to adiponectin expression in the colonic mucosa of murine colitis. C57BL/6 mice were feeding with 2% DSS for inducing colitis and using specially designed multiple platform water baths for sleep deprivation. Immuno-histochemical staining and Western blot analysis was performed using anti-adiponectin antibody. The expression level of adiponectin in mucosa was decreased in colitis, with the lowest level observed in colitis combined with sleep deprivation. Melatonin injection significantly (P < 0.05) recovered the expression of adiponectin. This study suggests that melatonin and adiponectin synergistically potentiate the anti-inflammatory effects in murine colitis.
