Published online Jun 14, 2016. doi: 10.3748/wjg.v22.i22.5254
Peer-review started: March 3, 2016
First decision: March 31, 2016
Revised: April 22, 2016
Accepted: May 4, 2016
Article in press: May 4, 2016
Published online: June 14, 2016
AIM: To detect the expression of the long noncoding RNA HOTAIR in colon cancer and analyze its relationship with clinicopathological parameters of colon cancer.
METHODS: Total RNA was extracted from 80 colon cancer tissues and matched tumor-adjacent normal colon tissues and reverse transcribed. Quantitative polymerase chain reaction was used to detect the expression of HOTAIR. The relationship between the expression of HOTAIR and clinicopathological parameters of colon cancer was analyzed.
RESULTS: The expression of HOTAIR was significantly higher in colon cancer tissues than in matched tumor-adjacent normal colon tissues (P < 0.05). HOTAIR expression was significantly higher in cases with lymph node metastasis than in those without metastasis; in lowly differentiated and undifferentiated cases than in highly and moderately differentiated cases; and in stages III + IV cases than in stages I + II cases (P < 0.05).
CONCLUSION: HOTAIR expression is upregulated in colon cancer, suggesting that HOTAIR plays an important role in the tumorigenesis, development and metastasis of colon cancer. HOTAIR may act as an oncogene and represents a new molecular target for the treatment of colon cancer.
Core tip: This study aimed to detect the expression of HOTAIR in colon cancer and analyze its relationship with clinicopathological parameters of colon cancer. Total RNA was extracted from 80 colon cancer tissues and matched tumor-adjacent normal colon tissues and reverse transcribed. HOTAIR expression was upregulated in colon cancer, suggesting that it may play an important role in the tumorigenesis, development and metastasis of colon cancer. HOTAIR might acts as an oncogene and could be a new molecular target for the treatment of colon cancer.