Observational Study
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World J Gastroenterol. Nov 14, 2014; 20(42): 15780-15786
Published online Nov 14, 2014. doi: 10.3748/wjg.v20.i42.15780
Autoimmune gastritis presenting as iron deficiency anemia in childhood
Cristina Gonçalves, Maria Emília Oliveira, Ana M Palha, Anabela Ferrão, Anabela Morais, Ana Isabel Lopes
Cristina Gonçalves, Ana Isabel Lopes, Gastroenterology Unit, Pediatric Department, University Hospital Santa Maria, Lisbon Academic Medical Centre, 1649-035 Lisboa, Portugal
Maria Emília Oliveira, Ana M Palha, Pathology Department, University Hospital Santa Maria, Lisbon Academic Medical Centre, 1649-035 Lisboa, Portugal
Anabela Ferrão, Anabela Morais, Hematology Unit , Pediatric Department, University Hospital Santa Maria, Lisbon Academic Medical Centre, 1649-035 Lisboa, Portugal
Author contributions: Gonçalves C, Ferrão A, Morais A and Lopes AI observed the patients and collected patient and laboratorial data; Lopes AI performed esophagogastroduodenoscopies and biopsy sample collection; Palha AM and Oliveira ME performed the histopathological analysis; Gonçalves C and Lopes AI wrote the paper.
Correspondence to: Ana Isabel Lopes, MD, PhD, Gastroenterology Unit, Pediatric Department, University Hospital Santa Maria, Lisbon Academic Medical Centre, 1649-035 Lisboa, Portugal. anaisalopes@sapo.pt
Telephone: +351-91-9075306 Fax: +351-91-9075306
Received: December 31, 2013
Revised: March 10, 2014
Accepted: April 28, 2014
Published online: November 14, 2014
Processing time: 321 Days and 10.8 Hours
Abstract

AIM: To characterize clinical, laboratorial, and histological profile of pediatric autoimmune gastritis in the setting of unexplained iron deficiency anemia investigation.

METHODS: A descriptive, observational study including pediatric patients with a diagnosis of autoimmune gastritis (positive parietal cell antibody and gastric corpus atrophy) established in a 6 year period (2006-2011) in the setting of refractory iron deficiency anemia (refractoriness to oral iron therapy for at least 6 mo and requirement for intravenous iron therapy) investigation, after exclusion of other potentially contributing causes of anemia. Helicobacter pylori (H. pylori) infection and anti-secretory therapy were also excluded. Data were retrospectively collected from clinical files, including: demographic data (age, gender, and ethnic background), past medical history, gastrointestinal symptoms, familial history, laboratorial evaluation (Hb, serum ferritin, serum gastrin, pepsinogen I/ pepsinogen II, B12 vitamin, intrinsic factor autoantibodies, thyroid autoantibodies, and anti-transglutaminase antibodies), and endoscopic and histological findings (HE, Periodic Acid-Schiff/Alcian blue, gastrin, chromogranin A and immunochemistry analysis for CD3, CD20 and CD68). Descriptive statistical analysis was performed (mean, median, and standard deviation).

RESULTS: We report a case-series concerning 3 girls and 2 boys with a mean age of 13.6 ± 2.8 years (3 Caucasian and 2 African). One girl had type I diabetes. Familial history was positive in 4/5 cases, respectively for autoimmune thyroiditis (2/5), sarcoidosis (1/5) and multiple myeloma (1/5). Laboratorial evaluation on admission included: Hb: 9.5 ± 0.7 g/dL; serum ferritin: 4.0 ± 0.9 ng/mL; serum gastrin: 393 ± 286 pg/mL; low pepsinogen I/ pepsinogen II ratio in 1/5 patients; normal vitamin B12 levels (analyzed in 3 patients). Endoscopy findings included: duodenal nodularity (2/5) and gastric fold softening (2/5), and histological evaluation showed corpus atrophic gastritis with lymphocytic infiltration (5/5), patchy oxyntic gland mononuclear cell infiltration (5/5), intestinal and/or pseudo-pyloric metaplasia in corpus mucosa (4/5), and enterochromaffin cell hyperplasia (4/5). Immunochemistry for gastrin on corpus biopsies was negative in all cases. Duodenal histology was normal. All biopsies were negative for H. pylori (Giemsa staining and cultural examination).

CONCLUSION: We highlight autoimmune gastritis as a diagnosis to be considered when investigating refractory iron deficiency anemia in children, particularly in the setting of a personal/familial history of autoimmune disease, as well as the diagnostic contribution of a careful immunohistological evaluation.

Keywords: Autoimmune gastritis; Iron deficiency anemia; Children

Core tip: Autoimmune gastritis (AIG) is a rare entity at young age. Although classically associated with pernicious anemia in adulthood, its presentation as iron-deficiency anemia (IDA) has been recently reported, particularly in younger patients. Our study aimed to further contribute to a better characterization of clinical and histological expression of AIG at pediatric age, highlighting IDA as a precocious hematological manifestation and the diagnostic contribution of a careful immunohistological evaluation. Furthermore, in the setting of personal and/or familial history of autoimmune disease, the importance of taking AIG into account and including parietal cell antibodies in the autoimmune screening panel is emphasized.