Retrospective Study
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World J Gastroenterol. Sep 7, 2014; 20(33): 11840-11849
Published online Sep 7, 2014. doi: 10.3748/wjg.v20.i33.11840
Identification of differential proteins in colorectal cancer cells treated with caffeic acid phenethyl ester
Yu-Jun He, Wan-Ling Li, Bao-Hua Liu, Hui Dong, Zhi-Rong Mou, Yu-Zhang Wu
Yu-Jun He, Wan-Ling Li, Hui Dong, Zhi-Rong Mou, Yu-Zhang Wu, Institute of Immunology of PLA, the Third Military Medical University, Chongqing 400038, China
Yu-Jun He, Bao-Hua Liu, Department of General Surgery, Daping Hospital and Research Institute of Surgery, the Third Military Medical University, Chongqing 400042, China
Author contributions: He YJ and Mou ZR designed the research; He YJ, Li WL and Dong H performed the research; He YJ, Liu BH and Mou ZR analyzed the data; He YJ, Liu BH, Mou ZR and Wu YZ wrote the paper; Mou ZR and Wu YZ contributed equally to this work.
Supported by National Natural Science Foundation of China No. 30872466 and No. 30801096, the Natural Science Foundation of Chongqing No. 2011BB5032, and PLA Logistics Science Research during the 12th Five-Year Plan Period No. BWS11J041
Correspondence to: Zhi-Rong Mou, PhD, Institute of Immunology of PLA, the Third Military Medical University, Chongqing 400038, China. mouzr@yahoo.com
Telephone: +86-23-68752680 Fax: +86-23-68752789
Received: January 2, 2014
Revised: March 18, 2014
Accepted: April 28, 2014
Published online: September 7, 2014
Processing time: 247 Days and 17 Hours
Abstract

AIM: To investigate the molecular mechanisms of the anti-cancer activity of caffeic acid phenethyl ester (CAPE).

METHODS: Protein profiles of human colorectal cancer SW480 cells treated with or without CAPE were analysed using a two-dimensional (2D) electrophoresis gel-based proteomics approach. After electrophoresis, the gels were stained with Coomassie brilliant blue R-250. Digital images were taken with a GS-800 Calibrated Densitometer, and image analysis was performed using PDQuest 2-D Analysis software. The altered proteins following CAPE treatment were further identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry following a database search. The identified proteins were validated by Western blot and immunofluorescence assay.

RESULTS: CAPE induced human colorectal cancer cell apoptosis. Four up-regulated proteins and seven down-regulated proteins in colorectal cancer cells treated with CAPE were found. The identified down-regulated proteins in CAPE-treated colorectal cancer cells were Triosephosphate Isomerase (Tim), Proteasome subunit alpha 4 (PSMA4) protein, Guanine nucleotide binding protein beta, Phosphoserine aminotransferase 1 (PSAT1), PSMA1, Myosin XVIIIB and Tryptophanyl-tRNA synthetase. Notably, CAPE treatment led to the down-regulation of PSAT1 and PSMA1, two proteins that have been implicated in tumorigenesis. The identified up-regulated proteins were Annexin A4, glyceraldehyde-3-phosphate dehydrogenase, Glucosamine-6-phosphate deaminase 1 (GNPDA1), and Glutathione peroxidase (GPX-1). Based on high match scores and potential role in cell growth control, PSMA1, PSAT1, GNPDA1 and GPX-1 were further validated by Western blotting and immunofluorescence assay. PSMA1 and PSAT1 were down-regulated, while GNPDA1 and GPX-1 were up-regulated in CAPE-treated colorectal cancer cells.

CONCLUSION: These differentiated proteins in colorectal cancer cells following CAPE treatment, may be potential molecular targets of CAPE and involved in the anti-cancer effect of CAPE.

Keywords: Caffeic acid phenethyl ester; Colorectal cancer; Proteomics; Two-dimensional electrophoresis; Mass spectrometry

Core tip: To investigate the molecular mechanisms of the anti-cancer activity of caffeic acid phenethyl ester (CAPE), CAPE-treated colorectal cancer SW480 cells were analysed by a 2D-gel based proteomics approach. Four up-regulated proteins and seven down-regulated proteins in CAPE-treated SW480 cells were found and further identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry following a database search. The down-regulated proteins, PSMA1 and PSAT1 and up-regulated proteins GNPDA1 and GPX-1 were validated by Western blotting. The two tumorigenesis associated proteins, PSMA1 and PSAT1, were further confirmed by immunofluorescence assay. These differentiated proteins in colorectal cancer cells following CAPE treatment, may be potential molecular targets of CAPE and involved in the anti-cancer effect of CAPE.