Dai YC, Zhu XS, Nan QZ, Chen ZX, Xie JP, Fu YK, Lin YY, Lian QN, Sang QF, Zhan XJ. Identification of differential gene expressions in colorectal cancer and polyp by cDNA microarray. World J Gastroenterol 2012; 18(6): 570-575 [PMID: 22363125 DOI: 10.3748/wjg.v18.i6.570]
Corresponding Author of This Article
Yi-Chen Dai, Professor, Department of Gastroenterology, The 174th Hospital of the Chinese PLA, Wenyuan Road, Xiamen 361003, Fujian Province, China. dyichen@sina.com
Article-Type of This Article
Brief Article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Yi-Chen Dai, Xiao-San Zhu, Zhang-Xin Chen, Jun-Pei Xie, Yu-Ka Fu, Yuan-Yuan Lin, Qing-Na Lian, Qiao-Fang Sang, Xiao-Juan Zhan, Department of Gastroenterology, Chenggong Hospital, Xiamen University, Xiamen 361003, Fujian Province, China
Yi-Chen Dai, Xiao-San Zhu, Zhang-Xin Chen, Jun-Pei Xie, Yu-Ka Fu, Yuan-Yuan Lin, Qing-Na Lian, Qiao-Fang Sang, Xiao-Juan Zhan, Department of Gastroenterology, The 174th Hospital of the Chinese PLA, Wenyuan Road, Xiamen 361003, Fujian Province, China
Qing-Zhen Nan, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
Author contributions: Zhu XS and Lin YY performed the majority of experiments; Nan QZ, Zhan XJ and Chen ZX provided vital reagents and analytical tools and were also involved in editing the manuscript; Xie JP, Fu YK, Lian QN and Sang QF coordinated and provided the collection of all the human material in addition to providing financial support for this work; Dai YC and Zhu XS designed the study and wrote the manuscript.
Supported by Grant from Medical Technology Innovation Project of Nanjing Military, No. 09MA066
Correspondence to: Yi-Chen Dai, Professor, Department of Gastroenterology, The 174th Hospital of the Chinese PLA, Wenyuan Road, Xiamen 361003, Fujian Province, China. dyichen@sina.com
Telephone: +86-592-6335702 Fax: +86-592-6335702
Received: May 31, 2011 Revised: July 17, 2011 Accepted: August 15, 2011 Published online: February 14, 2012
Abstract
AIM: To screen the differential expressed genes in colorectal cancer and polyp tissue samples.
METHODS: Tissue specimens containing 16 cases of colorectal adenocarcinoma and colorectal polyp vs normal mucosae were collected and subjected to cDNA microarray and bioinformatical analyses. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to confirm some of the cDNA microarray data.
RESULTS: The experimental data showed that eight genes were differentially expressed, most of which were upregulated in adenomatous polyp lesions. Forty-six genes expressions were altered in colorectal cancers, of which 29 were upregulated and 17 downregulated, as compared to the normal mucosae. In addition, 18 genes were similarly altered in both adenomatous polyps and colorectal cancer. qRT-PCR analyses confirmed the cDNA microarray data for four of those 18 genes: MTA1, PDCD4, TSC1 and PDGFRA.
CONCLUSION: These differentially expressed genes likely represent biomarkers for early detection of colorectal cancer and may be potential therapeutic targets after confirmed by further studies.
