Vujanac A, Jakovljevic V, Djordjevic D, Zivkovic V, Stojkovic M, Celikovic D, Andjelkovic N, Skevin AJ, Djuric D. Nitroglycerine effects on portal vein mechanics and oxidative stress in portal hypertension. World J Gastroenterol 2012; 18(4): 331-339 [PMID: 22294839 DOI: 10.3748/wjg.v18.i4.331]
Corresponding Author of This Article
Vladimir Jakovljevic, Professor, Department of Physiology, Faculty of Medicine, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, Serbia. drvladakgbg@yahoo.com
Article-Type of This Article
Brief Article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Jan 28, 2012; 18(4): 331-339 Published online Jan 28, 2012. doi: 10.3748/wjg.v18.i4.331
Nitroglycerine effects on portal vein mechanics and oxidative stress in portal hypertension
Andreja Vujanac, Vladimir Jakovljevic, Dusica Djordjevic, Vladimir Zivkovic, Mirjana Stojkovic, Dragan Celikovic, Nebojsa Andjelkovic, Aleksandra Jurisic Skevin, Dragan Djuric
Andreja Vujanac, Vladimir Jakovljevic, Dusica Djordjevic, Vladimir Zivkovic, Department of Physiology, Faculty of Medicine, University of Kragujevac, 34000 Kragujevac, Serbia
Mirjana Stojkovic, Department of Gastroenterology and Echosonography Unit, Clinical Centre of Belgrade, 11000 Belgrade, Serbia
Dragan Celikovic, Nebojsa Andjelkovic, Internal Clinic, Clinical Centre “Kragujevac”, Faculty of Medicine, University of Kragujevac, 34000 Kragujevac, Serbia
Aleksandra Jurisic Skevin, Department of Physical Medicine and Rehabilitation, Faculty of Medicine, University of Kragujevac, 34000 Kragujevac, Serbia
Dragan Djuric, Institute of Medical Physiology, Belgrade University School of Medicine, 11000 Belgrade, Serbia
Author contributions: Jakovljevic V and Djuric D designed the study, were involved in editing the manuscript and peformed supervision under all study issues; Vujanac A and Djordjevic D wrote the manuscript; Zivkovic V performed biochemical analysis and coordinated between experimental and clinical issues of the study; Jurisic Skevin A performed statistical analysis; Celikovic D, Andjelkovic N and Stojkovic M performed ultrasonographical examinations.
Supported by The Grant from the Ministry of Science and Technical Development of the Republic of Serbia, No. 175043
Correspondence to: Vladimir Jakovljevic, Professor, Department of Physiology, Faculty of Medicine, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, Serbia. drvladakgbg@yahoo.com
Telephone: +381-34-342944 Fax: +381-34-306800
Received: May 28, 2011 Revised: September 2, 2011 Accepted: November 9, 2011 Published online: January 28, 2012
Abstract
AIM: Тo examine the effects of nitroglycerine on portal vein haemodynamics and oxidative stress in patients with portal hypertension.
METHODS: Thirty healthy controls and 39 patients with clinically verified portal hypertension and increased vascular resistance participated in the study. Liver diameters, portal diameters and portal flow velocities were recorded using color flow imaging/pulsed Doppler detection. Cross-section area, portal flow and index of vascular resistance were calculated. In collected blood samples, superoxide anion radical (O2-), hydrogen peroxide (H2O2), index of lipid peroxidation (measured as TBARS) and nitric oxide (NO) as a marker of endothelial response (measured as nitrite-NO2-) were determined. Time-dependent analysis was performed at basal state and in 10th and 15th min after nitroglycerine (sublingual 0.5 mg) administration.
RESULTS: Oxidative stress parameters changed significantly during the study. H2O2 decreased at the end of study, probably via O2- mediated disassembling in Haber Weiss and Fenton reaction; O2- increased significantly probably due to increased diameter and tension and decreased shear rate level. Consequently O2- and H2O2 degradation products, like hydroxyl radical, initiated lipid peroxidation. Increased blood flow was to some extent lower in patients than in controls due to double paradoxes, flow velocity decreased, shear rate decreased significantly indicating non Newtonian characteristics of portal blood flow.
CONCLUSION: This pilot study could be a starting point for further investigation and possible implementation of some antioxidants in the treatment of portal hypertension.
