She JJ, Zhang PG, Wang X, Che XM, Wang ZM. Side population cells isolated from KATO III human gastric cancer cell line have cancer stem cell-like characteristics. World J Gastroenterol 2012; 18(33): 4610-4617 [PMID: 22969237 DOI: 10.3748/wjg.v18.i33.4610]
Corresponding Author of This Article
Jun-Jun She, PhD, Department of General Surgery, First Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China. sjuns@sina.com
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World J Gastroenterol. Sep 7, 2012; 18(33): 4610-4617 Published online Sep 7, 2012. doi: 10.3748/wjg.v18.i33.4610
Side population cells isolated from KATO III human gastric cancer cell line have cancer stem cell-like characteristics
Jun-Jun She, Peng-Ge Zhang, Xuan Wang, Xiang-Ming Che, Zi-Ming Wang
Jun-Jun She, Xiang-Ming Che, Department of General Surgery, First Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Peng-Ge Zhang, Department of Mathematics, Xidian University, Xi’an 710061, Shaanxi Province, China
Xuan Wang, Department of Endocrinology, First Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Zi-Ming Wang, Department of Urology, Second Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
Author contributions: She JJ, Che XM, Wang ZM made substantial contributions to conception and design; She JJ performed the majority experiment and drafted the article, revised it critically for important intellectual content; Wang X performed partial experiments and revised the manuscript; Zhang PG did the data analysis; all authors approved the version to be published.
Supported by National Natural Science Foundation of China, No. 81072108 and No. 30600615; China Postdoctoral Science Foundation and No. 20090450167 and No. 201003676; PhD Programs Fund of the Chinese Ministry of Education, No. 20090201120068; International Cooperation Program of Shaanxi Province, No. 2012KW-38; Science and Technology Program of Shaanxi Province, No. 2010K14-02; and Basic Research Funds for the Central Universities
Correspondence to: Jun-Jun She, PhD, Department of General Surgery, First Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China. sjuns@sina.com
Telephone: +86-29-85323870 Fax: +86-29-88202132
Received: August 8, 2011 Revised: February 29, 2012 Accepted: June 8, 2012 Published online: September 7, 2012
Abstract
AIM: To investigate whether the side population (SP) cells possess cancer stem cell-like characteristics in vitro and the role of SP cells in tumorigenic process in gastric cancer.
METHODS: We analyzed the presence of SP cells in different human gastric carcinoma cell lines, and then isolated and identified the SP cells from the KATO III human gastric cancer cell line by flow cytometry. The clonogenic ability and self-renewal were evaluated by clone and sphere formation assays. The related genes were determined by reverse transcription polymerase chain reaction. To compare tumorigenic ability, SP and non-side population (NSP) cells from the KATO III human gastric cancer cell line were subcutaneously injected into nude mice.
RESULTS: SP cells from the total population accounted for 0.57% in KATO III, 1.04% in Hs-746T, and 0.02% in AGS (CRL-1739). SP cells could grow clonally and have self-renewal capability in conditioned media. The expression of ABCG2, MDRI, Bmi-1 and Oct-4 was different between SP and NSP cells. However, there was no apparent difference between SP and NSP cells when they were injected into nude mice.
CONCLUSION: SP cells have some cancer stem cell-like characteristics in vitro and can be used for studying the tumorigenic process in gastric cancer.
