Xu YF, Yu SN, Lu ZH, Liu JP, Chen J. Fascin promotes the motility and invasiveness of pancreatic cancer cells. World J Gastroenterol 2011; 17(40): 4470-4478 [PMID: 22110277 DOI: 10.3748/wjg.v17.i40.4470]
Corresponding Author of This Article
Dr. Jie Chen, Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tsinghua University, Beijing 100730, China. xhblk@163.com
Article-Type of This Article
Original Article
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World J Gastroenterol. Oct 28, 2011; 17(40): 4470-4478 Published online Oct 28, 2011. doi: 10.3748/wjg.v17.i40.4470
Fascin promotes the motility and invasiveness of pancreatic cancer cells
Yan-Feng Xu, Shuang-Ni Yu, Zhao-Hui Lu, Jian-Ping Liu, Jie Chen
Yan-Feng Xu, Shuang-Ni Yu, Zhao-Hui Lu, Jian-Ping Liu, Jie Chen, Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tsinghua University, Beijing 100730, China
Author contributions: Xu YF and Yu SN contributed equally to this work; Xu YF performed the majority of experiments and drafted the manuscript; Yu SN edited and revised the manuscript and was involved in data interpretation; Lu ZH and Liu JP contributed to the literature review; Chen J designed and supervised the research and gave funding support.
Supported by Grants from the Doctoral Fund from the Ministry of Education of China, No. 20060023013; the National Nature Science Foundation of China, No. 30471970 and 30973470; the National Science and Technology Support Project (the 11th Five-Year Plan) of China, No. 2006BAI02A14; the Scientific Research Special Projects of Health Ministry of China, No. 200802011; the National Data Sharing Project in Human Health, No. 2005DKA32403; Roche Company
Correspondence to: Dr. Jie Chen, Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tsinghua University, Beijing 100730, China. xhblk@163.com
Telephone: +86-10-65295490 Fax: +86-10-65295490
Received: February 22, 2011 Revised: June 8, 2011 Accepted: June 15, 2011 Published online: October 28, 2011
Abstract
AIM: To explore the role of actin-bundling protein, fascin during the progression of pancreatic cancer.
METHODS: The plasmid expressing human fascin-1 was stably transfected into the pancreatic cancer cell line MIA PaCa-2. The proliferation, cell cycle, motility, scattering, invasiveness and organization of the actin filament system in fascin-transfected MIA PaCa-2 cells and control non-transfected cells were determined.
RESULTS: Heterogeneous overexpression of fascin markedly enhanced the motility, scattering, and invasiveness of MIA PaCa-2 cells. However, overexpression of fascin had minimal effect on MIA PaCa-2 cell proliferation and cell cycle. In addition, cell morphology and organization of the actin filament system were distinctly altered in fascin overexpressed cells. When transplanted into BALB/c-nu mice, fascin-transfected pancreatic cancer cells developed solid tumors at a slightly slower rate, but these tumors displayed more aggressive behavior in comparison with control tumors.
CONCLUSION: Fascin promotes pancreatic cancer cell migration, invasion and scattering, thus contributes to the aggressive behavior of pancreatic cancer cells.
