Wang JX, He YL, Zhu ST, Yang S, Zhang ST. Aberrant methylation of the 3q25 tumor suppressor gene PTX3 in human esophageal squamous cell carcinoma. World J Gastroenterol 2011; 17(37): 4225-4230 [PMID: 22072855 DOI: 10.3748/wjg.v17.i37.4225]
Corresponding Author of This Article
Shu-Tian Zhang, Professor, Department of Gastroenterology, Beijing Friendship Hospital Affiliated to the Capital Medical University, No. 95 Yong’an Road, Xuanwu District, Beijing 100050, China. jxwang88@gmail.com
Article-Type of This Article
Brief Article
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Jun-Xiong Wang, Medical and Health Center, Beijing Friendship Hospital Affiliated to the Capital Medical University, Beijing 100050, China
Yuan-Long He, Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao 266071, Shandong Province, China
Shu-Tian Zhang, Sheng-Tao Zhu, Department of Gastroenterology, Beijing Friendship Hospital Affiliated to the Capital Medical University, Beijing 100050, China
Shuo Yang, Laboratory Medicine Department, Peking University Third Hospital, Beijing 100050, China
Author contributions: Wang JX and He YL performed the majority of the experiments; Zhu ST and Yang S provided vital reagents, analytical tools and were also involved in editing the manuscript; Zhang ST designed the study; Wang JX and He YL contributed equally to this study.
Supported by National High Technology Research and Development Program of China (863 Program), No.2007AA02Z4Z4; China Postdoctoral Science Foundation, No.20090460394 and Beijing Municipal Natural Science Foundation, No.7072022
Correspondence to: Shu-Tian Zhang, Professor, Department of Gastroenterology, Beijing Friendship Hospital Affiliated to the Capital Medical University, No. 95 Yong’an Road, Xuanwu District, Beijing 100050, China. jxwang88@gmail.com
Telephone: +86-10-63138702 Fax: +86-10-63138076
Received: December 23, 2010 Revised: March 24, 2011 Accepted: March 31, 2011 Published online: October 7, 2011
Abstract
AIM: To identify the novel methylation-silenced gene pentraxin 3 (PTX3) in esophageal squamous cell carcinoma (ESCC).
METHODS: PTX3 mRNA expression was examined in six human ESCC cell lines, one human immortalized normal esophageal epithelial cell line, primary ESCC tumor tissue, and paired adjacent nontumor tissue using reverse transcription polymerase chain reaction (RT-PCR). Semi-quantitative immunohistochemistry was used to examine cellular localisation and protein levels. Methylation specific PCR and bisulphite genomic sequencing were employed to investigate the methylation of the candidate gene.
RESULTS: In the majority of ESCC cell lines, we found that PTX3 expression was down-regulated due to gene promoter hypermethylation, which was further confirmed by bisulphite genomic sequencing. Demethylation treatment with 5-aza-2’-deoxycytidine restored PTX3 mRNA expression in ESCC cell lines. Methylation was more common in tumor tissues (85%) than in adjacent nontumor tissues (25%) (P < 0 .01).
CONCLUSION: PTX3 is down-regulated through promoter hypermethylation in ESCC, and could potentially serve as a biomarker of ESCC.
