Brief Article
Copyright ©2011 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Aug 28, 2011; 17(32): 3739-3744
Published online Aug 28, 2011. doi: 10.3748/wjg.v17.i32.3739
Sleeve gastrectomy prevents lipoprotein receptor-1 expression in aortas of obese rats
Jie Bai, Yong Wang, Yuan Liu, Dong-Hua Geng, Jin-Gang Liu
Jie Bai, Yong Wang, Yuan Liu, Dong-Hua Geng, Jin-Gang Liu, Department of General Surgery, China Medical University and the affiliated Shengjing Hospital, Shenyang 110004, Liaoning Province, China
Author contributions: Bai J conducted the experiments and prepared the manuscript; Wang Y revised the manuscript; Liu Y and Geng DH conducted the experiments; Liu JG designed the research, conducted the experiments, prepared the manuscript and supervised the project.
Supported by A Grant from the National Natural Science Foundation of China, No. 81000158 and the Special Fund for Technology of Liaoning Province, No. L2010591
Correspondence to: Jin-Gang Liu, Professor of Medicine, Chief, Department of General Surgery, China Medical University and the affiliated Shengjing Hospital, 36 Sanhao Street, Shenyang 110004, Liaoning Province, China. wangy54321@gmail.com
Telephone: +86-24-23144851 Fax: +86-24-23144851
Received: September 24, 2010
Revised: March 29, 2011
Accepted: April 5, 2011
Published online: August 28, 2011
Abstract

AIM: To investigate the effects of sleeve gastrectomy on adipose tissue infiltration and lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) expression in rat aortas.

METHODS: Twenty-four rats were randomized into three groups: normal chow (control), high fat diet (HD) and high fat diet with sleeve gastrectomy (SG). After surgery, the HD and SG groups were fed a high fat diet. Animals were sacrificed and plasma high density lipoprotein (HDL) and low density lipoprotein (LDL) levels were determined. LOX-1 protein and LOX-1 mRNA expression was also measured. Aortas were stained with Nile red to visualize adipose tissue.

RESULT: Body weights were higher in the HD group compared to the other groups. HDL levels in control, HD, and SG groups were 32.9 ± 6.2 mg/dL, 43.4 ± 4.0 mg/dL and 37.5 ± 4.3 mg/dL, respectively. LDL levels in control, HD, and SG groups were 31.8 ± 4.5 mg/dL, 53.3 ± 5.1 mg/dL and 40.5 ± 3.7 mg/dL, respectively. LOX-1 protein and LOX-1 mRNA expression was greater in the HD group versus the other groups. Staining for adipose tissue in aortas was greater in the HD group in comparison to the other groups. Thus, a high fat diet elevates LOX-1 protein and mRNA expression in aorta.

CONCLUSION: Sleeve gastrectomy decreases plasma LDL levels, and downregulates LOX-1 protein and mRNA expression.

Keywords: Sleeve gastrectomy; Morbid obesity; High fat diet; Aorta; Lipoprotein receptor-1 expression