Brief Article
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World J Gastroenterol. Dec 21, 2010; 16(47): 6026-6034
Published online Dec 21, 2010. doi: 10.3748/wjg.v16.i47.6026
Prognostic values of chromosome 18q microsatellite alterations in stage II colonic carcinoma
Wei Wang, Guo-Qiang Wang, Xiao-Wei Sun, Gong Chen, Yuan-Fang Li, Li-Yi Zhang, Hai-Bo Qiu, Chun-Yu Huang, You-Qing Zhan, Zhi-Wei Zhou
Wei Wang, Guo-Qiang Wang, Xiao-Wei Sun, Yuan-Fang Li, Hai-Bo Qiu, Chun-Yu Huang, You-Qing Zhan, Zhi-Wei Zhou, Department of Gastric and Pancreatic Surgery, State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong Province, China
Gong Chen, Li-Yi Zhang, Department of Colorectal Surgery, State Key Laboratory of Oncology in South China, Sun Yat-Sen University, Cancer Center, Guangzhou 510060, Guangdong Province, China
Author contributions: Wang W and Wang GQ performed the majority of experiments and wrote the manuscript; Sun XW and Zhang LY performed the technical aspects of the experiments; Li YF, Qiu HB and Huang CY collected the samples; Zhan YQ, Zhou ZW and Chen G designed the study and provided financial support for this work.
Correspondence to: Zhi-Wei Zhou, Professor, Department of Gastric and Pancreatic Surgery, State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, 651 Dongfeng East Road, Guangzhou 510060, Guangdong Province, China. zhouzhw@sysucc.org.cn
Telephone: +86-20-87343626 Fax: +86-20-87343392
Received: May 24, 2010
Revised: July 6, 2010
Accepted: July 13, 2010
Published online: December 21, 2010
Abstract

AIM: To investigate the prognostic value of chromosome 18q microsatellite alterations (MA) in stage II colon cancer.

METHODS: One hundred and six patients with sporadic stage II colon cancer were enrolled in this study. DNA was extracted from formalin-fixed, paraffin-embedded tumor and adjacent normal mucosal tissue samples. MA, including loss of heterozygosity (LOH) and microsatellite instability (MSI), was analyzed by polymerase chain reaction, polyacrylamide gel-electrophoresis and DNA sequencing at 5 microsatellite loci on chromosome 18q (D18S474, D18S55, D18S58, D18S61 and D18S64).

RESULTS: Among the 102 patients eligible for MA information, the overall frequencies of LOH, high and low frequency MSI/microsatellite stable were 49.0%, 17.6% and 82.4%, respectively. The high frequency of 18q-LOH was significantly associated with the poor 5-year overall survival (OS) (P = 0.008) and disease free survival (P = 0.006). High levels of MSI were significantly associated with a longer 5-year OS (P = 0.045) while the higher frequency of 18q-LOH at the loci of D18S474 and D18S61 was significantly associated with a poorer 5-year OS (P = 0.010 and 0.005, respectively). But multivariate analysis showed that only the frequency of 18q-LOH was significantly associated with the prognosis of the disease.

CONCLUSION: High frequency of 18q-LOH is an independent prognostic factor indicating poor prognosis of the patients with stage II colon cancer.

Keywords: Chromosome 18q, Loss of heterozygosity, Microsatellite instability, Stage II colon cancer, Prognosis