Brief Article
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World J Gastroenterol. Dec 7, 2010; 16(45): 5759-5765
Published online Dec 7, 2010. doi: 10.3748/wjg.v16.i45.5759
Protective effects of Lactobacillus plantarum against epithelial barrier dysfunction of human colon cell line NCM460
Zhi-Hua Liu, Tong-Yi Shen, Peng Zhang, Yan-Lei Ma, Mary Pat Moyer, Huan-Long Qin
Zhi-Hua Liu, Peng Zhang, Yan-Lei Ma, Huan-Long Qin, Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China
Tong-Yi Shen, Department of Surgery, Shanghai Tenth People’s Hospital, Shanghai 200233, China
Mary Pat Moyer, INCELL Corporation, San Antonio, TX 78249, United States
Author contributions: Qin HL and Liu ZH conceived the study, participated in its design and coordination, acquired funding, and wrote the manuscript; Liu ZH and Shen TY conducted majority of the experiments, and statistical analyses; Moyer MP provided NCM460 cells and revised the manuscript; Zhang P and Ma YL performed the experiment of Western blotting; all authors read and approved the final manuscript.
Supported by The National Natural Science Foundation of China, No. 30672044; and the Ministry of Science and Technology of China, No. 2008CB517403
Correspondence to: Huan-Long Qin, MD, Professor, Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 600 Yishan Road, Shanghai 200233, China.
Telephone: +86-21-64369181 Fax: +86-21-64368920
Received: June 2, 2010
Revised: July 3, 2010
Accepted: July 10, 2010
Published online: December 7, 2010

AIM: To investigate the effects of Lactobacillus plantarum (L. plantarum) in the intestinal permeability and expression of tight junction (TJ) using the normal human colon cell line NCM460.

METHODS: Paracellular permeability of NCM460 monolayers was determined by transepithelial electrical resistance and dextran permeability. Expression of TJ proteins in NCM460 cell monolayers was detected by Western blotting and quantitative real-time polymerase chain reaction.

RESULTS: L. plantarum played an important role in increasing transepithelial electrical resistance and decreasing the permeability to macromolecules of NCM460 monolayers against the disruption caused by enteropathogenic Escherichia coli (E. coli) or enteroinvasive E. coli. L. plantarum also prevented the decrease in the expression of TJ proteins and F-actin in NCM460 cells.

CONCLUSION: L. plantarum can protect against dysfunction of NCM460 intestinal epithelial barrier caused by enteropathogenic E. coli or enteroinvasive E. coli, and thus can be a potential candidate of therapeutic agents for the treatment of intestinal diseases.

Keywords: Lactobacillus plantarum, NCM460, Tight junction, Intestinal barrier