Jaime SC, Maribel AM, Eliakym AM, José RN, Julio G, Laura SM, Rosalío RP. ApoB-100, ApoE and CYP7A1 gene polymorphisms in Mexican patients with cholesterol gallstone disease. World J Gastroenterol 2010; 16(37): 4685-4690 [PMID: 20872969 DOI: 10.3748/wjg.v16.i37.4685]
Corresponding Author of This Article
Ramos-Payán Rosalío, PhD, Laboratorio de Biología Molecular, Facultad de Ciencias Químico Biológicas, Doctorado en Biotecnología y Maestría en Ciencias Biomédicas, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa 80010, México. ramospayan@yahoo.com.mx
Article-Type of This Article
Brief Article
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Aguilar-Medina Maribel, Arámbula-Meraz Eliakym, Romero-Navarro José, Sicairos-Medina Laura, Ramos-Payán Rosalío, Facultad de Ciencias Químico Biológicas, Doctorado en Biotecnología y Maestría en Ciencias Biomédicas, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa 80010, México
Granados Julio, División de Inmunogenética, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, D.F., 14000, México
Author contributions: Jaime SC, Laura SM and Maribel AM performed the research; Jaime SC, Rosalío RP and Eliakym AM designed the research; José RN and Julio G analyzed the data; Rosalío RP wrote the paper.
Supported by PROFAPI-UAS and CECYT from Sinaloa, México
Correspondence to: Ramos-Payán Rosalío, PhD, Laboratorio de Biología Molecular, Facultad de Ciencias Químico Biológicas, Doctorado en Biotecnología y Maestría en Ciencias Biomédicas, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa 80010, México. ramospayan@yahoo.com.mx
Telephone: +52-667-7137860 Fax: +52-667-7137860
Received: May 13, 2010 Revised: June 29, 2010 Accepted: July 6, 2010 Published online: October 7, 2010
Abstract
AIM: To determine the possible association of the ApoB-100 (XbaI), ApoE (HhaI) and CYP7A1 (BsaI) gene polymorphisms, with the development of cholesterol gallstone disease (GD) in a Mexican population.
METHODS: The polymorphisms were analyzed by polymerase chain reaction followed by restriction fragment length polymorphism, in two groups matched by ethnicity, age and sex: patients with GD (n = 101) and stone-free control subjects (n = 101).
RESULTS: Allelic frequencies in patients and controls were: 34.16% vs 41.58% (P = 0.124) for X+ of ApoB-100; 4.46% vs 5.94% (P = 0.501) for E2, 85.64% vs 78.22% (P = 0.052) for E3, 9.90% vs 15.84% (P = 0.075) for E4 of ApoE; and 25.74% vs 27.72% (P = 0.653) for C of CYP7A1. Differences in genotypic frequencies between the studied groups were not significant (P < 0.05).
CONCLUSION: These results demonstrated that no association exists between the studied polymorphisms and cholelithiasis in this high prevalent population.
