Assumpção MB, Martins LC, Melo Barbosa HP, Barile KADS, Almeida SS, Assumpção PP, Corvelo TCO. Helicobacter pylori in dental plaque and stomach of patients from Northern Brazil. World J Gastroenterol 2010; 16(24): 3033-3039 [PMID: 20572307 DOI: 10.3748/wjg.v16.i24.3033]
Corresponding Author of This Article
Dr. Tereza Cristina de Oliveira Corvelo, Professor, MD, PhD, Immunogenetics Laboratory, Federal University of Pará, 66075-110, Belém, Pará, Brazil. tereza@ufpa.br
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Brief Article
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World J Gastroenterol. Jun 28, 2010; 16(24): 3033-3039 Published online Jun 28, 2010. doi: 10.3748/wjg.v16.i24.3033
Helicobacter pylori in dental plaque and stomach of patients from Northern Brazil
Mônica Baraúna Assumpção, Luisa Caricio Martins, Hivana Patricia Melo Barbosa, Katarine Antonia dos Santos Barile, Sintia Silva de Almeida, Paulo Pimentel Assumpção, Tereza Cristina de Oliveira Corvelo
Mônica Baraúna Assumpção, Endoscopy Department, João de Barros Barreto University Hospital, 66073-000, Belém, Pará, Brazil
Luisa Caricio Martins, Immunopathology Laboratory, Nucleus of Tropical Medicine, Federal University of Pará, 66075-000, Belém, Pará, Brazil
Hivana Patricia Melo Barbosa, Katarine Antonia dos Santos Barile, Sintia Silva de Almeida, Tereza Cristina de Oliveira Corvelo, Immunogenetics Laboratory, Federal University of Pará, 66075-110, Belém, Pará, Brazil
Paulo Pimentel Assumpção, Surgery Department, João de Barros Barreto University Hospital, 66073-000, Belém, Pará, Brazil
Author contributions: Assumpção MB, Corvelo TCO and Assumpção PP conceived and designed the research; Assumpção MB performed endoscopy and provided clinical advice, took part in the experiments and collected the samples; Melo Barbosa HP, Martins LC, Barile KAS and Almeida SS performed the majority of the experiments; Assumpção MB, Martins LC and Corvelo TCO supervised the project and wrote the paper; all authors read and approved the final version of manuscript.
Supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and Federal University of Pará
Correspondence to: Dr. Tereza Cristina de Oliveira Corvelo, Professor, MD, PhD, Immunogenetics Laboratory, Federal University of Pará, 66075-110, Belém, Pará, Brazil. tereza@ufpa.br
Telephone: +55-91-32248225 Fax: +55-91-32410032
Received: November 14, 2009 Revised: January 13, 2010 Accepted: January 20, 2010 Published online: June 28, 2010
Abstract
AIM: To establish whether virulence factor genes vacA and cagA are present in Helicobacter pylori (H. pylori) retrieved from gastric mucosa and dental plaque in patients with dyspepsia.
METHODS: Cumulative dental plaque specimens and gastric biopsies were submitted to histological examination, rapid urease test and polymerase chain reaction (PCR) assays to detect the presence of cagA and vacA polymorphisms.
RESULTS: Detection of H. pylori from dental plaque and gastric biopsy samples was greater by PCR compared to histological examination and the rapid urease test. DNA from H. pylori was detected in 96% of gastric mucosa samples and in 72% of dental plaque samples. Sixty-three (89%) of 71 dental plaque samples that were H. pylori-positive also exhibited identical vacA and cagA genotypes in gastric mucosa. The most common genotype was vacAs1bm1 and cagA positive, either in dental plaque or gastric mucosa. These virulent H. pylori isolates were involved in the severity of clinical outcome.
CONCLUSION: These pathogenic strains were found simultaneously in dental plaque and gastric mucosa, which suggests that gastric infection is correlated with the presence of H. pylori in the mouth.
