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Endoscopic submucosal dissection in dogs in a World Gastroenterology Organisation training center
Miguel A Tanimoto, Gonzalo Torres-Villalobos, Rikiya Fujita, Patricio Santillan-Doherty, Jorge Albores-Saavedra, German Gutierrez, Luis A Martin-del-Campo, Carlos Bravo-Reyna, Octavio Villanueva, Jose J Villalobos, Misael Uribe, Miguel A Valdovinos
Miguel A Tanimoto, Jose J Villalobos, Misael Uribe, Miguel A Valdovinos, Department of Gastroenterology and World Gastroenterology Organisation Training Center, National Institute of Medical Sciences and Nutrition Salvador Zubiran, Mexico City 14000, Mexico
Gonzalo Torres-Villalobos, Patricio Santillan-Doherty, Luis A Martin-del-Campo, Department of Surgery and Experimental Surgery, National Institute of Medical Sciences and Nutrition Salvador Zubiran, Mexico City 14000, Mexico
Rikiya Fujita, The Cancer Institute Hospital of JFCR Tokyo, Tokyo 135-8550, Japan
Jorge Albores-Saavedra, German Gutierrez, Department of Pathology, National Institute of Medical Sciences and Nutrition Salvador Zubiran, Mexico City 14000, Mexico
Carlos Bravo-Reyna, Department of Experimental Surgery, National Institute of Medical Sciences and Nutrition Salvador Zubiran, Mexico City 14000, Mexico
Octavio Villanueva, Animal lab and Department of Experimental Research, National Institute of Medical Sciences and Nutrition Salvador Zubiran, Mexico City 14000, Mexico
Author contributions: Tanimoto MA designed the study, performed the endoscopic procedures and prepared the manuscript; Fujita R, Villalobos JJ and Uribe M participated in the design of the study and facilitated the donation of instruments for the research; Torres-Villalobos G performed the surgical resection of the esophagus and stomach in the canine models; Santillan-Doherty P manages all the facilities at the experimental surgery department; Albores-Saavedra J and Gutierrez G were the expert pathologists who studied the specimens; Martin-del-Campo LA was the endoscopy assistant during the ESD procedures; Bravo-Reyna C and Villanueva O were the veterinarians that took care of and quarantined the dogs; Valdovinos MA is director of the WGO training center and, together with the authors, implemented the ESD training program.
Correspondence to: Dr. Miguel A Tanimoto, Department of Gastroenterology and World Gastroenterology Organisation Training Center, National Institute of Medical Sciences and Nutrition Salvador Zubiran, Vasco de Quiroga # 15, Colonia Seccion XVI, Delegacion Tlalpan C.P., Mexico City 14000, Mexico. matanimoto@prodigy.net.mx
Telephone: +52-55-25683552 Fax: +52-55-56665982
Received: November 7, 2009
Revised: January 18, 2010
Accepted: January 25, 2010
Published online: April 14, 2010
AIM: To evaluate if canine models are appropriate for teaching endoscopy fellows the techniques of endoscopic submucosal dissection (ESD).
METHODS: ESD was performed in 10 canine models under general anesthesia, on artificial lesions of the esophagus or stomach marked with coagulation points. After ESD, each canine model was euthanized and surgical resection of the esophagus or stomach was carried out according to “The Principles of Humane Experimental Technique, Russel and Burch”. The ESD specimens were fixed with needles on cork submerged in a formol solution with the esophagus or stomach, and delivered to the pathology department to be analyzed.
RESULTS: ESD was completed without complications using the Hook-knife in five esophageal areas, with a procedural duration of 124 ± 19 min, a length of 27.4 ± 2.6 mm and a width of 21 ± 2.4 mm. ESD was also completed without complications using the IT-knife2 in five gastric areas, with a procedural duration of 92.6 ± 19 min, a length of 32 ± 2.5 mm and a width of 18 ± 3.7 mm.
CONCLUSION: ESD is feasible in the normal esophagus and stomach of canine models, which are appropriate for teaching this technique.
