Brief Article
Copyright ©2009 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Nov 7, 2009; 15(41): 5176-5180
Published online Nov 7, 2009. doi: 10.3748/wjg.15.5176
Modification of sleep architecture in an animal model of experimental cirrhosis
Anabel Jiménez-Anguiano, Vanessa Díaz-Medina, Blanca Eugenia Farfán-Labonne, Gloria Giono-Chiang, David Kersenobich, Mario García-Lorenzana, Maria Concepción Gutiérrez-Ruiz, Javier Velázquez-Moctezuma
Anabel Jiménez-Anguiano, Vanessa Díaz-Medina, Mario García-Lorenzana, Javier Velázquez-Moctezuma, Area of Neuroscience, Department of Biology of Reproduction, Universidad Autónoma Metropolitana-Iztapalapa, CP 09340, Mexico City, Mexico
Blanca Eugenia Farfán-Labonne, Gloria Giono-Chiang, Maria Concepción Gutiérrez-Ruiz, Cell Physiology Laboratory, Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, CP 09340, Mexico City, Mexico
David Kersenobich, Unit of Liver, Pancreas and Motility, Faculty of Medicine, Universidad Nacional Autónoma de México, Mexico City, D.F CP 09340, Mexico
Author contributions: Jiménez-Anguiano A, Gutiérrez-Ruiz MC and Velázquez-Moctezuma J contributed equally to this work; Jiménez-Anguiano A, Gutiérrez-Ruiz MC, Velázquez-Moctezuma J, Giono-Chiang G, Kersenobich D and García-Lorenzana M designed the research and analyzed the data; Jiménez-Anguiano A, Díaz-Medina V, Farfán-Labonne BE and Giono-Chiang G performed the research.
Supported by Grant 50633 from CONACyT to Jiménez-Anguiano A
Correspondence to: Dr. Javier Velázquez-Moctezuma, Area of Neuroscience, Department of Biology of Reproduction, Universidad Autónoma Metropolitana-Iztapalapa, CP 09340, Mexico City, Mexico. jvm@xanum.uam.mx
Telephone: +52-55-8044704 Fax: +52-55-8044704
Received: April 21, 2009
Revised: September 21, 2009
Accepted: September 28, 2009
Published online: November 7, 2009
Abstract

AIM: To analyze the polygraphic sleep patterns during cirrhosis progression in a rat model by repeated CCl4 administration.

METHODS: Male Wistar rats received three weekly injections of CCl4 for 11 wk, and were analyzed before and during the induction of cirrhosis. Rats were implanted with electrodes to record their sleep patterns. Polygraph recordings were made weekly over 11 wk for 8 h, during the light period. After a basal recording, rats received three weekly injections of CCl4. Histological confirmation of cirrhosis was performed after 11 wk.

RESULTS: The results showed a progressive decrease in total wake time that reached statistical significance from the second week of treatment. In addition, there was an increase in total time of slow wave sleep (SWS) II and rapid eye movement sleep (REM sleep) in most of the 11 wk. SWS I showed no significant variations. During the final weeks, a significant increase in REM sleep frequency was also observed. Histological analyses of the livers showed unequivocal signs of cirrhosis.

CONCLUSION: These data suggest that hepatic failure produced by CCl4 administration is capable of modifying the sleep pattern even after only a few doses.

Keywords: Experimental cirrhosis; Sleep; Rapid eye movement sleep; CCl4; Wakefulness