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World J Gastroenterol. Jun 14, 2009; 15(22): 2800-2804
Published online Jun 14, 2009. doi: 10.3748/wjg.15.2800
Expression of semaphorin 5A and its receptor plexin B3 contributes to invasion and metastasis of gastric carcinoma
Guo-Qing Pan, Hong-Zheng Ren, Shu-Fang Zhang, Xi-Mei Wang, Ji-Fang Wen
Guo-Qing Pan, Hong-Zheng Ren, Ji-Fang Wen, Department of Pathology, Xiangya Medical College, Central South University, Changsha 410008, Hunan Province, China
Guo-Qing Pan, Department of Pathology, the first Affiliated Hospital of Kunming Medical College, Kunming 530032, Yunnan Province, China
Shu-Fang Zhang, Central Laboratory, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, Haikou 570208, Hainan Province, China
Xi-Mei Wang, Department of Basic Medicine, Huaihua Medical College, Huaihua 418000, Hunan Province, China
Author contributions: Pan GQ and Ren HZ contributed equally to this work; Pan GQ designed the research and wrote the manuscript; Ren HZ analyzed the data by Western blotting and RT-PCR; Zhang SF dealt with the statistical data; Wang XM collected the samples; Wen JF revised the manuscript.
Correspondence to: Ji-Fang Wen, Professor, Department of Pathology, Xiangya Medical College, Central South University, Changsha 410008, Hunan Province, China. jifangwen@hotmail.com
Telephone: +86-731-2650400
Fax: +86-731-2650400
Received: October 23, 2008
Revised: May 5, 2009
Accepted: May 12, 2009
Published online: June 14, 2009
Abstract

AIM: To investigate the protein and mRNA expression of semaphorin 5A and its receptor plexin B3 in gastric carcinoma and explore its role in the invasion and metastasis of gastric carcinoma.

METHODS: Expression of semaphorin 5A and its receptor plexin B3 in 48 samples of primary gastric carcinoma, its corresponding non-neoplastic mucosa, and matched regional lymph node metastasis was assayed by reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR and Western blotting.

RESULTS: The protein and mRNA expression of semaphorin 5A and its receptor plexin B3 increased gradually in non-neoplastic mucosa, primary gastric carcinoma and lymph node metastasis (P < 0.05). Moreover, the expression of semaphorin 5A was closely correlated with that of plexin B3.

CONCLUSION: Semaphorin 5A and its receptor plexin B3 play an important role in the invasion and metastasis of gastric carcinoma.

Keywords: Semaphorin 5A; Plexin B3; Gastric carcinoma; Invasion; Metastasis