Orignal Articles
Copyright ©2009 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Jun 14, 2009; 15(22): 2714-2722
Published online Jun 14, 2009. doi: 10.3748/wjg.15.2714
Higher CO2-insufflation pressure inhibits the expression of adhesion molecules and the invasion potential of colon cancer cells
Jun-Jun Ma, Bo Feng, Yi Zhang, Jian-Wen Li, Ai-Guo Lu, Ming-Liang Wang, Yuan-Fei Peng, Wei-Guo Hu, Fei Yue, Min-Hua Zheng
Jun-Jun Ma, Bo Feng, Jian-Wen Li, Ai-Guo Lu, Ming-Liang Wang, Yuan-Fei Peng, Wei-Guo Hu, Fei Yue, Min-Hua Zheng, Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Minimally Invasive Surgery Center, Shanghai 200025, China
Jun-Jun Ma, Bo Feng, Yi Zhang, Yuan-Fei Peng, Fei Yue, Min-Hua Zheng, Shanghai Institute of Digestive Surgery, Shanghai 200025, China
Author contributions: Ma JJ carried out the Adhesion assays, drafted the manuscript and participated in the design of the study; Feng B carried out the molecular expression studies, and in vitro pneumoperitoneum establishment; Zhang Y carried out the cell invasive assay; Li JW participate in the induction of intra-abdominal tumors; Lu AG participated in the molecular expression studies; Wang ML performed the statistical analysis; Peng YF, Hu WG, Yue F participated in the cell invasive assay; Zheng MH conceived of the study, and participated in its design and coordination and helped to draft the manuscript.
Correspondence to: Min-Hua Zheng, Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Minimally Invasive Surgery Center, Shanghai 200025, China. marsnew790620@163.com
Telephone: +86-21-64458887
Fax: +86-21-64333548
Received: March 22, 2009
Revised: May 5, 2009
Accepted: May 12, 2009
Published online: June 14, 2009
Abstract

AIM: To investigate the influence of CO2-insufflation pressure on adhesion, invasion and metastatic potential of colon cancer cells based on adhesion molecules expression.

METHODS: With an in vitro artificial pneumoperitoneum model, SW1116 human colon carcinoma cells were exposed to CO2-insufflation in 5 different pressure groups: 6 mmHg, 9 mmHg, 12 mmHg, 15 mmHg and control group, respectively for 1 h. Expression of E-cadherin, ICAM-1, CD44 and E-selectin was measured at 0, 12, 24, 48 and 72 h after CO2-insufflation using flow cytometry. The adhesion and invasion capacity of SW1116 cells before and after exposure to CO2-insufflation was detected by cell adhesion/invasion assay in vitro. Each group of cells was injected intraperitoneally into 16 BALB/C mice. The number of visible abdominal cavity tumor nodules, visceral metastases and survival of the mice were recorded in each group.

RESULTS: The expression of E-cadherin, ICAM-1, CD44 and E-selectin in SW1116 cells were changed significantly following exposure to CO2 insufflation at different pressures (P < 0.05). The expression of E-cadherin, CD44 and ICAM-1 decreased with increasing CO2-insufflation pressure. The adhesive/invasive cells also decreased gradually with increasing pressure as determined by the adhesion/invasion assay. In animal experiments, the number of abdominal cavity tumor nodules in the 15 mmHg group was also significantly lower than that in the 6 mmHg group (29.7 ± 9.91 vs 41.7 ± 14.90, P = 0.046). However, the survival in each group was not statistically different.

CONCLUSION: CO2-insufflation induced a temporary change in the adhesion and invasion capacity of cancer cells in vitro. Higher CO2-insufflation pressure inhibited adhesion, invasion and metastatic potential in vitro and in vivo, which was associated with reduced expression of adhesion molecules.

Keywords: Adhesion molecule, Colorectal cancer, Metastasis, Pneumoperitoneum, Artificial, Tumor invasion