Orignal Articles
Copyright ©2009 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. May 14, 2009; 15(18): 2220-2227
Published online May 14, 2009. doi: 10.3748/wjg.15.2220
Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs
Ying Peng, Zhuo Wang, Yuan Lu, Chun-Fu Wu, Jing-Yu Yang, Xiao-Bo Li
Ying Peng, Zhuo Wang, Xiao-Bo Li, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200030, China
Ying Peng, Chun-Fu Wu, Jing-Yu Yang, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning Province, China
Yuan Lu, Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, NE2 4AA, United Kingdom
Author contributions: Li XB, Wu CF, Yang JY designed the research; Peng Y, Wang Z, and Li XB performed the majority of experiments; Peng Y, Li XB, and Lu Y wrote the paper.
Correspondence to: Xiao-Bo Li, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200030, China. xbli@sjtu.edu.cn
Telephone: +86-21-34204806
Fax: +86-21-34204804
Received: October 17, 2008
Revised: March 11, 2009
Accepted: March 18, 2009
Published online: May 14, 2009

AIM: To find a rapid and efficient analysis method of gastrointestinal microflora in Pi-deficient (spleen-deficient) rats and to evaluate traditional Chinese drugs.

METHODS: Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) based assay was performed to examine changes of intestinal microflora in two Pi-deficienct animal models and to evaluate the efficacy of four traditional Chinese drugs as well as a probiotic recipe and another therapy in Pi-deficient rats.

RESULTS: A molecular marker was identified for Pi-deficiency in rats. The pharmacodynamic evaluation system, including identified molecular markers (net integral area and abundance of DNA bands), Shannon’s index for diversity of intestinal microflora, and Sorenson’s pairwise similarity coefficient, was established. The four major clinical recipes of traditional Chinese drugs for Pi-deficiency in rats, especially at their medium dose (equivalence to the clinical dose), produced more pronounced recovery activities in Pi-deficient rats, while higher doses of these recipes did not show a better therapeutic effect but some toxic effects such as perturbation deterioration of intestinal microflora.

CONCLUSION: Both fingerprint analysis and identified marker can show Pi-deficiency in rats and its difference after treatment. The identified molecular marker may be applied in screening for the active compounds both in relative traditional Chinese drugs and in pharmacodynamic study of Pi-deficiency in rats.

Keywords: Pi-deficiency, Enterobacterial repetitive intergenic consensus-PCR, Traditional Chinese medicine