Li W, Li JF, Qu Y, Chen XH, Qin JM, Gu QL, Yan M, Zhu ZG, Liu BY. Comparative proteomics analysis of human gastric cancer. World J Gastroenterol 2008; 14(37): 5657-5664 [PMID: 18837081 DOI: 10.3748/wjg.14.5657]
Corresponding Author of This Article
Bing-Ya Liu, Professor, Shanghai Key Laboratory of Diagnosis and Treatment for Gastrointestinal Cancer, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. liubyrj@yahoo.com.cn
Article-Type of This Article
Gastric Cancer
Open-Access Policy of This Article
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World J Gastroenterol. Oct 7, 2008; 14(37): 5657-5664 Published online Oct 7, 2008. doi: 10.3748/wjg.14.5657
Comparative proteomics analysis of human gastric cancer
Wei Li, Jian-Fang Li, Ying Qu, Xue-Hua Chen, Jian-Min Qin, Qin-Long Gu, Min Yan, Zheng-Gang Zhu, Bing-Ya Liu
Wei Li, Jian-Fang Li, Ying Qu, Xue-Hua Chen, Qin-Long Gu, Min Yan, Zheng-Gang Zhu, Bing-Ya Liu, Shanghai Key Laboratory of Diagnosis and Treatment for Gastrointestinal Cancer, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
Wei Li, Jian-Min Qin, Putuo District Cancer Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200025, China
Author contributions: Liu BY designed the research; Li W, Li JF, Qu Y, Chen XH performed research; Yan M, Gu QL, Zhu ZG, and Qin JM supported the clinical samples and clinical data; Li W and Liu BY wrote the paper.
Supported by National High Technology Research and Development Program of China (863 Program) No. 2006AA 02A301 and No. 2007AA02Z179; Science and Technology Commission of Shanghai Municipality, No. 07jc14041; National Key Fundamental Research 973 Project, No. 2002CB13700
Correspondence to: Bing-Ya Liu, Professor, Shanghai Key Laboratory of Diagnosis and Treatment for Gastrointestinal Cancer, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. liubyrj@yahoo.com.cn
Telephone: +86-21-64674654 Fax: +86-21-64373909
Received: May 26, 2008 Revised: August 26, 2008 Accepted: September 2, 2008 Published online: October 7, 2008
Abstract
AIM: To isolate and identify differentially expressed proteins between cancer and normal tissues of gastric cancer by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).
METHODS: Soluble fraction proteins of gastric cancer tissues and paired normal tissues were separated by 2-DE. The differentially expressed proteins were selected and identified by MALDI-TOF-MS and database search.
RESULTS: 2-DE profiles with high resolution and reproducibility were obtained. Twenty-three protein spots were excised from sliver staining gel and digested in gel by trypsin, in which fifteen protein spots were identified successfully. Among the identified proteins, there were ten over-expressed and five under-expressed proteins in stomach cancer tissues compared with normal tissues.
CONCLUSION: In this study, the well-resolved, reproducible 2-DE patterns of human gastric cancer tissue and paired normal tissue were established and optimized and certain differentially-expressed proteins were identified. The combined use of 2-DE and MS provides an effective approach to screen for potential tumor markers.
