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Copyright ©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. Sep 21, 2008; 14(35): 5428-5431
Published online Sep 21, 2008. doi: 10.3748/wjg.14.5428
Distribution of secretory inhibitor of platelet microbicidal protein among anaerobic bacteria isolated from stool of children with diarrhea
Iuri B Ivanov, Viktor A Gritsenko
Iuri B Ivanov, Viktor A Gritsenko, Department of Human Microbiology, Institute of Cellular and Intracellular Symbiosis, Russian Academy of Sciences, Pionerskaya st., 11, Orenburg 460000, Russia
Author contributions: Ivanov IB and Gritsenko VA contributed equally to this work.
Supported by The Russian Foundation of Basic Research and Government of Orenburg region, No. 07-04-97624 and No. 08-04-99105
Correspondence to: Dr. Iuri B Ivanov, Department of Human Microbiology, Institute of Cellular and Intracellular Symbiosis, Russian Academy of Sciences, Volgogradskaya st., 36/3, of .93, Orenburg 460056, Russia. mil-for@rambler.ru
Telephone: +7-35-32775417 Fax: +7-35-32774463
Received: January 31, 2008
Revised: July 29, 2008
Accepted: August 5, 2008
Published online: September 21, 2008
Abstract

AIM: To study the secretory inhibitor of platelet microbicidal protein (SIPMP) phenotypes of faecal anaerobic isolates from patients with diarrhea.

METHODS: Faecal isolates of anaerobic bacteria (B. fragilis, n = 42; B. longum, n = 70; A. israelii, n = 21; E. lentum, n = 12) from children with diarrhea were tested. SIPMP production was tested by inhibition of platelet microbicidal protein (PMP) bioactivity against B. subtilis and was expressed as percentage of inhibition of PMP bactericidal activity.

RESULTS: Among anaerobic isolates 80% of B. longum strains, 85.7% of A. israelii strains, 50% of E. lentum strains and 92.86% of B. fragilis strains were SIPMP-positive. The isolated anaerobic organisms demonstrated SIPMP production at a mean level of 13.8% ± 0.7%, 14.7% ± 1.8%, 3.9% ± 0.9% (P < 0.05) and 26.8% ± 7.5% (P < 0.05) for bifidobacteria, A. israelii, E. lentum and B. fragilis, respectively.

CONCLUSION: Data from the present study may have significant implications in understanding the pathogenesis of microecological disorders in the intestine, as well as for future improvement in the prevention and therapy of anaerobe-associated infections.

Keywords: Platelet microbicidal protein; Secretory inhibitor; Anaerobic bacteria; Intestine