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World J Gastroenterol. Aug 14, 2008; 14(30): 4810-4815
Published online Aug 14, 2008. doi: 10.3748/wjg.14.4810
Gastric cancer cell lines induced by trichostatin A
Xiao-Ming Zou, Yun-Long Li, Hao Wang, Wu Cui, Xiao-Lin Li, Song-Bin Fu, Hong-Chi Jiang
Xiao-Ming Zou, Yun-Long Li, Hao Wang, Wu Cui, Xiao-Lin Li, Department of General Surgery, the Second Clinical College, Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Song-Bin Fu, Laboratory of Medical Genetics, Harbin Medical University, Harbin 150081, Heilongjiang Province, China
Hong-Chi Jiang, Department of General Surgery, the First Clinical College, Harbin Medical University, Harbin 150081, Heilongjiang Province, China
Author contributions: Zou XM and Li YL contributed equally to this work; Zou XM, Li YL, Fu SB and Jiang HC designed the research; Li YL, Wang H, Cui W, and Li XL carried out the project; Li YL, Wang H and Cui W analyzed the data; Li YL and Li XL wrote the paper.
Correspondence to: Dr. Yun-Long Li, Department of General Surgery, the Second Clinical College, Harbin Medical University, Harbin 150086, Heilongjiang Province, China. hydeyll.student@sina.com
Telephone: +86-451-86605126 Fax: +86-451-86605126
Received: May 12, 2008
Revised: July 19, 2008
Accepted: July 26, 2008
Published online: August 14, 2008
Abstract

AIM: To explore the effect of trichostatin A (TSA) on apoptosis and acetylated histone H3 levels in gastric cancer cell lines BGC-823 and SGC-7901.

METHODS: The effect of TSA on growth inhibition and apoptosis was examined by MTT, fluorescence microscopy and PI single-labeled flow cytometry. The acetylated histone H3 level was detected by Western blot.

RESULTS: TSA induced apoptosis in gastric cancer cell lines BGC-823 and SGC-7901 was in a dose and time-dependent manner. Apoptotic cells varied significantly between TSA treated groups (37.5 ng/mL 72 h for BGC-823 cell line and 75 ng/mL 72 h for SGC-7901 cell line) and control group (0.85 ± 0.14 vs 1.14 ± 0.07, P = 0.02; 0.94 ± 0.07 vs 1.15 ± 0.06, P = 0.02). Morphologic changes of apoptosis, including nuclear chromatin condensation and fluorescence strength, were observed under fluorescence microscopy. TSA treatment in BGC-823 and SGC-7901 cell lines obviously induced cell apoptosis, which was demonstrated by the increased percentage of sub-G1 phase cells, the reduction of G1-phase cells and the increase of apoptosis rates in flow cytometric analysis. The result of Western blot showed that the expression of acetylated histone H3 increased in BGC-823 and SGC-7901 TSA treatment groups as compared with the control group.

CONCLUSION: TSA can induce cell apoptosis in BGC-823 and SGC-7901 cell lines. The expression of acetylated histone H3 might be correlated with apoptosis.

Keywords: BGC-823, SGC-7901, Trichostatin A, Apoptosis, Acetylated histone H3, Gastric cancer