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Copyright ©2008 The WJG Press and Baishideng. All rights reserved.
World J Gastroenterol. May 28, 2008; 14(20): 3249-3253
Published online May 28, 2008. doi: 10.3748/wjg.14.3249
Proteasome inhibitor ameliorates severe acute pancreatitis and associated lung injury of rats
Xi Chen, Shun-Le Li, Tao Wu, Ji-Dong Liu
Xi Chen, Shun-Le Li, Tao Wu, Ji-Dong Liu, Department of General Surgery, The Second Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
Author contributions: Chen X and Li SL contributed equally to this work; Chen X and Li SL designed the research; Li SL and Liu JD performed the research; Wu T contributed to new reagents/analytic tools; Chen X, Li SL and Liu JD analyzed the data; Chen X and Li SL wrote the paper.
Correspondence to: Xi Chen, Department of General Surgery, The Second Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China. 2002chenxi@163.com
Telephone: +86-29-87679278
Received: November 18, 2007
Revised: February 27, 2008
Accepted: March 5, 2008
Published online: May 28, 2008
Abstract

AIM: To observe the effect of proteasome inhibitor MG-132 on severe acute pancreatitis (SAP) and associated lung injury of rats.

METHODS: Male adult SD rats were randomly divided into SAP group, sham-operation group, and MG-132 treatment group. A model of SAP was established by injection of 5% sodium taurocholate into the biliary-pancreatic duct of rats. The MG-132 group was pretreated with 10 mg/kg MG-132 intraperitoneally (ip) 30 min before the induction of pancreatitis. The changes in serum amylase, myeloperoxidase (MPO) activity of pancreatic and pulmonary tissue were measured. The TNF-α level in pancreatic cytosolic fractions was assayed with an enzyme-linked immunosorbent assay (ELISA) kit. Meanwhile, the pathological changes in both pancreatic and pulmonary tissues were also observed.

RESULTS: MG-132 significantly decreased serum amylase, pancreatic weight/body ratio, pancreatic TNF-α level, pancreatic and pulmonary MPO activity (P < 0.05). Histopathological examinations revealed that pancreatic and pulmonary samples from rats pretreated with MG-132 demonstrated milder edema, cellular damage, and inflammatory activity (P < 0.05).

CONCLUSION: The proteasome inhibitor MG-132 shows a protective effect on severe acute pancreatitis and associated lung injury of rats.

Keywords: Severe acute pancreatitis, Proteasome inhibitor, MG-132, Lung injury