Yu L, Zhang ZF, Jing CX, Wu FL. Intraperitoneal administration of gonadotropin-releasing hormone-PE40 induces castration in male rats. World J Gastroenterol 2008; 14(13): 2106-2109 [PMID: 18395915 DOI: 10.3748/wjg.14.2106]
Corresponding Author of This Article
Dr. Li Yu, Department of Biochemistry, Medical College, Jinan University, Guangzhou 510632, Guangdong Province, China. doctoryuli@yahoo.com.cn
Article-Type of This Article
Rapid Communication
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World J Gastroenterol. Apr 7, 2008; 14(13): 2106-2109 Published online Apr 7, 2008. doi: 10.3748/wjg.14.2106
Intraperitoneal administration of gonadotropin-releasing hormone-PE40 induces castration in male rats
Li Yu, Zhong-Fang Zhang, Chun-Xia Jing, Feng-Lin Wu
Li Yu, Feng-Lin Wu, Department of Biochemistry, Medical College, Jinan University, Guangzhou 510632, Guangdong Province, China
Zhong-Fang Zhang, Department of Physiology, Medical College, Shantou University, Shantou 515031, Guangdong Province, China
Chun-Xia Jing, Department of Epidemiology, Medical College, Jinan University, Guangzhou 510632, Guangdong Province, China
Author contributions: Yu L wrote the paper and organized the figures; Zhang ZF did the ELISA assays; Wu FL collected the statistical data; Jing CX did the histochemical staining and evaluated the pathological data.
Correspondence to: Dr. Li Yu, Department of Biochemistry, Medical College, Jinan University, Guangzhou 510632, Guangdong Province, China. doctoryuli@yahoo.com.cn
Telephone: +86-20-85220256
Fax: +86-20-85221343
Received: March 7, 2006 Revised: February 19, 2008 Published online: April 7, 2008
Abstract
AIM: To evaluate the long-term effects of gonadotropin-releasing hormone (GnRH)-based vaccine on levels of GnRH antibody and testosterone, and vaccine-induced immunocastration on sexual behavior of male rats.
METHODS: The rats were treated with GnRH-PE40 intraperitoneally every other day for 12 wk. GnRH antibody and testosterone level in rat blood were determined by ELISA and radioimmunoassay, respectively. Morphological changes in testes and sexual behavior of rats were evaluated.
RESULTS: GnRH-PE40 induced a high production in GnRH antibody, decreased the serum testosterone level, testis atrophy and sexual function in rats.
CONCLUSION: Intraperitoneal administration of GnRH-PE40 produces structural and functional castration of male rat reproductive system by inducing anti-GnRH antibody.
