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World J Gastroenterol. Feb 21, 2007; 13(7): 1123-1128
Published online Feb 21, 2007. doi: 10.3748/wjg.v13.i7.1123
Effect of parenteral and early intrajejunal nutrition on pancreatic digestive enzyme synthesis, storage and discharge in dog models of acute pancreatitis
Huan-Long Qin, Zhen-Dong Su, Lei-Guang Hu, Zai-Xian Ding, Qing-Tian Lin
Huan-Long Qin, Zhen-Dong Su, Lei-Guang Hu, Zai-Xian Ding, Qing-Tian Lin, Institute of Parenteral Nutrition and Enteral Nutrition, Department of Surgery, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Yishan Rd. 600, Shanghai 200233, China
Author contributions: All authors contributed equally to the work.
Supported by grant from the Morning Star Fund of Shanghai, China, No. 99QB14010
Correspondence to: Dr. Huan-Long Qin, Institute of Parenteral Nutrition and Enteral Nutrition, Department of Surgery, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Yishan Rd. 600, Shanghai 200233, China. hlqin@sjtu.edu.cn
Telephone: +86-21-64942226 Fax: +86-21-64368920
Received: October 17, 2006
Revised: December 27, 2006
Accepted: January 4, 2007
Published online: February 21, 2007
Abstract

AIM: To study the effect of early intrajejunal nutrition on enzyme-protein synthesis and secretion during acute pancreatitis.

METHODS: Fifteen dogs were randomly divided into parenteral nutrition (n = 7) and early intrajejunal nutrition groups (n = 8). An acute pancreatitis model was induced by injecting 5% sodium taurocholate and trypsin into the pancreas via the pancreatic duct. Intrajejunal nutrition was delivered with a catheter via a jejunostomy tube after the model was established for 24 h. On d 1 and 7 and at the beginning of nutritional support, radioactive tracing and electron microscopes were used to evaluate the enzyme-protein synthesis in acinar cells, the subcellular fractionation and the change in zymogen granules after 1.85 × 106 Bq L-3H phenylalanine was infused at 30, 60, 120, and 180 min.

RESULTS: The 3H radioactivity in pancreatic acinar cells reached its peak level at 60 min, and the contents in the early intrajejunal nutrition group were higher than those in the parenteral nutrition group, which were then decreased. The mean number and area of zymogen granules did not show any significant statistical difference in both groups on d 1 or on d 7 (P > 0.05).

CONCLUSION: Early intrajejunal nutrition might be effective in dogs with acute pancreatitis.

Keywords: Parenteral nutrition, Enteral nutrition, Digestive enzyme, Acute pancreatitis