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Copyright ©2007 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 7, 2007; 13(45): 6060-6065
Published online Dec 7, 2007. doi: 10.3748/wjg.v13.i45.6060
Effect of fluoxetine on depression-induced changes in the expression of vasoactive intestinal polypeptide and corticotrophin releasing factor in rat duodenum
Yong-Lan Huang, Jie-Ping Yu, Gao-Hua Wang, Zhen-Hua Chen, Qing Wang, Ling Xiao
Yong-Lan Huang, Gao-Hua Wang, Zhen-Hua Chen, Ling Xiao, Department of Mental Health Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Jie-Ping Yu, Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Qing Wang, Department of Gastroenterology, Wuhan University Zhongnan Hospital, Wuhan 430071, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Supported by Department of Mental Health Center and Department Gastroenterology of Renmin Hospital of Wuhan University, Hubei Province, China
Correspondence to: Professor Jie-Ping Yu, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan 430060, Hubei Province, China. luxiong100@21cn.com
Telephone: +86-27-63389801 Fax: +86-27-88072022
Received: July 4, 2007
Revised: August 27, 2007
Accepted: October 28, 2007
Published online: December 7, 2007

AIM: To investigate changes in vasoactive intestinal polypeptide (VIP) and corticotrophin releasing factor (CRF) in the plasma and duodenum of chronic stress-induced depressed rats and the effects of fluoxetine hydrochloride (fluoxetine) treatment on depression-induced changes in VIP and CRF.

METHODS: A Sprague-Dawley rat model of chronic stress-induced depression was produced. Thirty experimental rats were randomly divided into the following groups: control group, saline-treated depressed group, and fluoxetine-treated depressed group. Open-field testing was performed to assess the rats’ behavior. VIP and CRF levels in plasma were measured by ELISA. Immunofluorescence techniques combined with laser scanning confocal microscopy (LSCM) were used to investigate VIP and CRF expression in the duodenum.

RESULTS: The open-field behavior, both crossing and rearing, of depression model rats, decreased significantly compared with those of normal control rats over 5 min. Defecation times increased significantly. Compared to the control group, FITC fluorescence of duodenal CRF expression and plasma CRF levels in the depressed rats increased significantly (fluorescence intensity of duodenal CRF: 11.82 ± 2.54 vs 25.17 ± 4.63; plasma CRF: 11.82 ± 2.54 ng/L vs 25.17 ± 4.63 ng/L, P < 0.01), whereas duodenal VIP expression and plasma VIP levels decreased significantly (fluorescence intensity of duodenal VIP: 67.37 ± 18.90 vs 44.51 ± 16.37; plasma VIP: 67.37 ± 18.90 ng/L vs 44.51 ± 16.37 ng/L, P < 0.01). Fluoxetine improved depressed behavior, increased VIP expression and decreased CRF expression in plasma and the duodenal tissue of depressed rats.

CONCLUSION: Chronic stress can induce injury to the duodenum, accompanied by increasing CRF and decreasing VIP in the plasma and duodenum. Treatment with fluoxetine can ameliorate pathological changes in the duodenum of depressed rats, which suggests that antidepressants are an effective therapeutic agent for some duodenal diseases caused by chronic stress. VIP is a potential therapeutic strategy.

Keywords: Depression, Plasma, Duodenum, Rat, Vasoactive intestinal polypeptide, Corticotrophin releasing factor, Fluoxetine hydrochloride