H Pylori
Copyright ©2007 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 21, 2007; 13(39): 5196-5207
Published online Oct 21, 2007. doi: 10.3748/wjg.v13.i39.5196
A novel phenol-bound pectic polysaccharide from Decalepis hamiltonii with multi-step ulcer preventive activity
BM Srikanta, MN Siddaraju, SM Dharmesh
BM Srikanta, MN Siddaraju, Shylaja M Dharmesh, Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore -570020, Karnataka, India
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Shylaja M Dharmesh, Department of Biochemistry and Nutrition, CFTRI, Mysore -570020, Karnataka, India. cancerbiolab@gmail.com
Telephone: +91-821-2514876 Fax: +91-821-2517233
Received: March 17, 2007
Revised: April 26, 2007
Accepted: July 15, 2007
Published online: October 21, 2007

AIM: To investigate H+, K+-ATPase inhibition, anti-H pylori, antioxidant, and the in vivo antiulcer potential of a pectic polysaccharide from Swallow root (Decalepis hamiltonii; SRPP).

METHODS: SRPP, with known sugar composition [rhamnose: arabinose: xylose: galactose in the ratio of 16:50:2:32 (w/w), with 141 mg/g of uronic acid] was examined for anti-ulcer potency in vivo against swim/ethanol stress-induction in animal models. Ulcer index, antioxidant/antioxidant enzymes, H+, K+-ATPase and gastric mucin levels were determined to assess the anti-ulcer potency. Anti-H pylori activity was also determined by viable colony count and electron microscopic studies.

RESULTS: SRPP, containing phenolics at 0.12 g GAE/g, prevented stress-induced gastric ulcers in animal models by 80%-85%. Down regulation of gastric mucin 2-3 fold, antioxidant/antioxidant enzymes and upregulation of 3 fold of H+, K+-ATPase in ulcerous animals were normalized upon treatment with SRPP. Histopathological analysis revealed protection to the disrupted gastric mucosal layer and epithelial glands. SRPP also inhibited H+, K+-ATPase in vitro, at an IC50 of 77 μg/mL as opposed to that of 19.3 μg/mL of Lansoprazole and H pylori growth at Minimum Inhibitory Concentration (MIC) of 150 μg/mL. In addition, free radical scavenging (IC50-40 μg/mL) and reducing power (3200 U/g) activities were also observed.

CONCLUSION: SRPP, with defined sugar composition and phenolics, exhibited multi-potent free radical scavenging, antioxidant, anti-H pylori, inhibition of H+, K+-ATPase and gastric mucosal protective activities. In addition, SRPP is non-toxic as opposed to other known anti-ulcer drugs, and therefore may be employed as a potential alternative for ulcer management.

Keywords: Swallow root pectic polysaccharide, Gastric ulcer, H+, K+-ATPase, Histopathology, Antioxidant activity, H pylori