Gastric Cancer
Copyright ©2007 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 7, 2007; 13(25): 3466-3471
Published online Jul 7, 2007. doi: 10.3748/wjg.v13.i25.3466
COX-2 expression in gastric cancer and its relationship with angiogenesis using tissue microarray
Xiao-Yun Mao, Xiao-Ge Wang, Xiao-Jun Lv, Lei Xu, Cheng-Bo Han
Xiao-Yun Mao, Xiao-Ge Wang, Lei Xu, The Fourth Affiliated Hospital, China Medical University, Shenyang 110000, Liaoning Province, China
Xiao-Jun Lv, Liaoning Province Tumor Hospital, Shenyang 110000, Liaoning Province, China
Cheng-Bo Han, Department of Oncology, Sheng Jing Hospital, China Medical University, Shenyang 110022, Liaoning Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Cheng-Bo Han, Department of Oncology, Sheng Jing Hospital, China Medical University, Shenyang 110022, Liaoning Province, China. hanchengbo@cmu2h.com
Telephone: +86-24-25943301
Received: January 7, 2007
Revised: January 9, 2007
Accepted: March 28, 2007
Published online: July 7, 2007
Abstract

AIM: To explore the expression and clinicopathological significance of cyclooxygenase-2 (COX-2) and microvessel density (MVD) in gastric carcinogenesis, and to investigate their roles in the invasion and the relationship between biological behaviors and prognosis of gastric cancer.

METHODS: Using Envision immunohistochemistry, COX-2 and CD34 expressions in gastric cancer tissue array were examined. MVD was counted and the relationship between the biological behaviors and prognosis was analyzed.

RESULTS: The expression of COX-2 in gastric cancer tissue was significantly higher than that in normal mucosa (χ2 = 12.191, P < 0.05). The over-expression of COX-2 in gastric cancer was obviously related to metastasis and depth of invasion (χ2 = 6.315, P < 0.05), but not related to the histological type and Borrmann type (χ2 = 5.391 and χ2 = 2.228, respectively). Moreover, MVD in gastric cancer tissues was significantly higher than that in the normal mucosa (65.49 ± 20.64 vs 36.21 ± 18.47, t/F = 7.53, P < 0. 05). MVD was related to the histologic type and metastasis (t/F = 3.68 and t/F = 4.214, respectively, P < 0. 05), but not related to the depth of invasion and Borrmann type (t/F = 0.583 and t/F = 0.459, respectively). MVD in COX-2-positive tissues was markedly higher compared to COX-2-negative tissues, indicating a positive correlation between COX-2 expression and MVD (t = 13.12, P < 0. 05).

CONCLUSION: Tissue microarray (TMA) is a powerful tool for rapid identification of the molecular alterations in gastric cancer. COX-2 expression, via inducing angiogenesis, may play an important role in gastric carcinogenesis. It could be served as a determinant factor for clinical prognosis and curative effect.

Keywords: Gastric cancer; Tissue microarray; COX-2; Immunohistochemistry; CD34; Microvessel density