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World J Gastroenterol. Jun 28, 2007; 13(24): 3354-3358
Published online Jun 28, 2007. doi: 10.3748/wjg.v13.i24.3354
Frequent loss of heterozygosity at 8p22 chromosomal region in diffuse type of gastric cancer
Hedayat Allah Hosseini, Ali Ahani, Hamid Galehdari, Ali Mohammad Froughmand, Masoud Hosseini, Abdolrahim Masjedizadeh, Mohammad Reza Zali
Hedayat Allah Hosseini, Abdolrahim Masjedizadeh, Biochemistry Department, Medical school, Ahwaz Jondishapoor University of Medical Science, Ahwaz, Iran
Ali Ahani, Masoud Hosseini, Biology Department, Shahid Beheshti University, Tehran, Iran.
Hamid Galehdari, Ali Muhammad Froughmand, Genetics Department, Shahid Chamran University, Ahwaz, Iran Mohammad Reza Zali, Research Center of Gastroenterology and Liver Disease, Shahid Beheshti University of Medical Science, Tehran, Iran
Author contributions: All authors contributed equally to the work.
Supported by Research Center of Gastric and Liver Disease in Tehran Taleghani hospital
Correspondence to: Hedayat Allah Hosseini, Biochemistry Department, Ahwaz Jondishapoor University of Medical Science, Ahwaz, Iran. hosseini.h@aujums.ac.ir
Telephone: +98-61-13332036 Fax: +98-61-13332036
Received: December 25, 2006
Revised: January 20, 2007
Accepted: January 25, 2007
Published online: June 28, 2007

AIM: To study the loss of heterozygosity (LOH) at 8p21-23 locus in diffuse gastric cancer.

METHODS: To evaluate the involvement of this region in gastric cancer, we used eight microsatellite markers covering two Mb of mentioned region, to perform a high-resolution analysis of allele loss in 42 cases of late diffuse gastric adenocarcinoma.

RESULTS: Six of these STS makers: D8S1149, D8S1645, D8S1643, D8S1508, D8S1591, and D8S1145 showed 36%, 28%, 37%, 41%, 44% and 53% LOH, respectively.

CONCLUSION: A critical region of loss, close to the NAT2 locus and relatively far from FEZ1 gene currently postulated as tumor suppressor gene in this region.

Keywords: Loss of heterozygosity, Tumor suppressor genes, diffuse type of gastric cancer, STS marker, N-Acetyltransferase 2, Fez1