Belmonte L, Coëffier M, Pessot FL, Miralles-Barrachina O, Hiron M, Leplingard A, Lemeland JF, Hecketsweiler B, Daveau M, Ducrotté P, Déchelotte P. Effects of glutamine supplementation on gut barrier, glutathione content and acute phase response in malnourished rats during inflammatory shock. World J Gastroenterol 2007; 13(20): 2833-2840 [PMID: 17569119 DOI: 10.3748/wjg.v13.i20.2833]
Corresponding Author of This Article
Professor Pierre Déchelotte, ADEN - Faculté de Médecine-Pharmacie de Rouen, 22 Bld Gambetta, 76183 Rouen, France. pierre.dechelotte@chu-rouen.fr
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Rapid Communication
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World J Gastroenterol. May 28, 2007; 13(20): 2833-2840 Published online May 28, 2007. doi: 10.3748/wjg.v13.i20.2833
Effects of glutamine supplementation on gut barrier, glutathione content and acute phase response in malnourished rats during inflammatory shock
Liliana Belmonte, Moïse Coëffier, Florence Le Pessot, Olga Miralles-Barrachina, Martine Hiron, Antony Leplingard, Jean-François Lemeland, Bernadette Hecketsweiler, Maryvonne Daveau, Philippe Ducrotté, Pierre Déchelotte
Liliana Belmonte, Moïse Coëffier, Olga Miralles-Barrachina, Antony Leplingard, Bernadette Hecketsweiler, Philippe Ducrotté, Pierre Déchelotte, Appareil Digestif Environnement Nutrition, ADEN EA3234, Institut Hospitalo-Universitaire de Recherche Biomédicale and Institut Fédératif de Recherches Multidisciplinaires sur les Peptides, Faculté de Médecine-Pharmacie, Rouen, France
Florence Le Pessot, Service d’Anatomie Pathologique, Centre Hospitalier Universitaire, Rouen, France
Martine Hiron, Maryvonne Daveau, INSERM unit 519, Institut Hospitalo-Universitaire de Recherche Biomédicale and Institut Fédératif de Recherches Multidisciplinaires sur les Peptides, Faculté de Médecine-Pharmacie, Rouen, France
Jean-François Lemeland, Groupe de Recherche sur les Antimicrobiens et les Microorganismes, GRAM EA2656, Institut Hospitalo-Universitaire de Recherche Biomédicale and Institut Fédératif de Recherches Multidisciplinaires sur les Peptides, Faculté de Médecine-Pharmacie, Rouen, France
Author contributions: All authors contributed equally to the work.
Correspondence to: Professor Pierre Déchelotte, ADEN - Faculté de Médecine-Pharmacie de Rouen, 22 Bld Gambetta, 76183 Rouen, France. pierre.dechelotte@chu-rouen.fr
Telephone: +33-2-32886465 Fax: +33-2-32888332
Received: March 2, 2007 Revised: March 20, 2007 Accepted: March 28, 2007 Published online: May 28, 2007
Abstract
AIM: To evaluate the effect of glutamine on intestinal mucosa integrity, glutathione stores and acute phase response in protein-depleted rats during an inflammatory shock.
METHODS: Plasma acute phase proteins (APP), jejunal APP mRNA levels, liver and jejunal glutathione concentrations were measured before and one, three and seven days after turpentine injection in 4 groups of control, protein-restricted, protein-restricted rats supplemented with glutamine or protein powder. Bacterial translocation in mesenteric lymph nodes and intestinal morphology were also assessed.
RESULTS: Protein deprivation and turpentine injection significantly reduced jejunal villus height, and crypt depths. Mucosal glutathione concentration significantly decreased in protein-restricted rats. Before turpentine oil, glutamine supplementation restored villus heights and glutathione concentration (3.24 ± 1.05 vs 1.72 ± 0.46 μmol/g tissue, P < 0.05) in the jejunum, whereas in the liver glutathione remained low. Glutamine markedly increased jejunal α1-acid glycoprotein mRNA level after turpentine oil but did not affect its plasma concentration. Bacterial translocation in protein-restricted rats was not prevented by glutamine or protein powder supplementation.
CONCLUSION: Glutamine restored gut glutathione stores and villus heights in malnourished rats but had no preventive effect on bacterial translocation in our model.
