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World J Gastroenterol. Apr 21, 2007; 13(15): 2218-2222
Published online Apr 21, 2007. doi: 10.3748/wjg.v13.i15.2218
Gene expression analysis of pancreatic cystic neoplasm in SV40Tag transgenic mice model
Jie Feng, Qiang Sun, Cheng Gao, Juan Dong, Xiao-Luan Wei, Hua Xing, Hou-Da Li
Jie Feng, Cheng Gao, Shanghai Laboratory Animal Research Center, Shanghai, 200032, China
Juan Dong, Hua Xing, Hou-Da Li, Comparative Medical Center, Yangzhou University, Yangzhou 225009, Jiangsu Province, China
Qiang Sun, Xiao-Luan Wei, Shanghai Institute of Brain Functional Genomics, East China Normal University, Shanghai 200062, China
Author contributions: All authors contributed equally to the work.
Supported by the National Key Technologies Research and Development Program of China during the 10th Five-Year Plan Period, No. 2001BA70113
Correspondence to: Dr. Qiang Sun, Shanghai Institute of Brain Functional Genomics, East China Normal University, Shanghai 200062, China. qsun@brain.ecnu.edu.cn
Telephone: +86-21-62235792 Fax: +86-21-62601953
Received: December 24, 2006
Revised: January 15, 2007
Accepted: February 8, 2007
Published online: April 21, 2007
Abstract

AIM: To study the gene expression changes in pancreatic cystic neoplasm in SV40Tag transgenic mice model and to provide information about the prevention, clinical diagnosis and therapy of pancreatic cancer.

METHODS: Using the pBC-SV40Tag transgenic mice model of pancreatic cystic neoplasm, we studied the gene expression changes by applying high-density microarrays. Validation of part gene expression profiling data was performed using real-time PCR.

RESULTS: By using high-density oligonucleotide microarray, of 14 113 genes, 453 were increased and 760 decreased in pancreatic cystic neoplasm, including oncogenes, cell-cycle-related genes, signal transduction-related genes, skeleton-related genes and metabolism-related genes. Among these, we confirmed the changes in Igf, Shh and Wnt signal pathways with real-time PCR. The results of real-time PCR showed similar expression changes in gene chip.

CONCLUSION: all the altered expression genes are associated with cell cycle, DNA damage and repair, signal pathway, and metabolism. SV40Tag may cooperate with several proteins in promoting tumorigenesis.

Keywords: SV40Tag, Pancreatic cystic neoplasm, cDNA microarray