Basic Research
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Feb 28, 2006; 12(8): 1225-1228
Published online Feb 28, 2006. doi: 10.3748/wjg.v12.i8.1225
Effect of hypercholesterolemia on experimental colonic anastomotic wound healing in rats
Meral Şen, A Ziya Anadol, Mehmet Oğuz
Meral Şen, Department of Surgery, School of Medicine, Fatih University, 06500 Ankara, Turkey
A Ziya Anadol, Department of Surgery, School of Medicine, Ondokuz Mayıs University, 55139 Samsun, Turkey
Mehmet Oğuz, Department of Surgery, School of Medicine, Gazi University, 06500 Ankara, Turkey
Co-first-author: Meral Şen and A Ziya Anadol
Correspondence to: Meral Sen, MD, Department of Surgery, School of Medicine, Fatih University, 06500 Ankara, Turkey.
Telephone: +90-312-2210620 Fax: +90-312-2213670
Received: August 16, 2005
Revised: September 15, 2005
Accepted: September 20, 2005
Published online: February 28, 2006

AIM: To evaluate the mechanical and biochemical parameters of colonic anastomotic healing in hypercholesterolemic rats.

METHODS: Sixty rats were divided into two groups of 30 each according to their dietary regimens. The test group was fed with a high cholesterol-containing diet for two months while the control group had standard diet. These two groups were further divided into three subgroups consisting of ten rats each. After hypercholesterolemia was established, left colon resection and anastomosis were performed in both groups and samples from liver and abdominal aorta were taken to evaluate the systemic effects of hypercholesterolemia. Anastomotic wound healing, blow-out pressures and tissue hydroxyproline levels were evaluated.

RESULTS: The test group had a significant weight gain in two months. Microscopic examination of the abdominal aorta revealed no atherosclerotic change in none of the groups, but liver tissue specimens showed significant steatosis in the test group. Tissue hydroxyproline levels and anastomotic blow-out pressures were significantly lower in the test group than in the controls.

CONCLUSION: Hypercholesterolemia not only increases hydroxyproline levels and blow-out pressures but also worsens anastomotic wound healing.

Keywords: Hypercholesterolemia, Colonic anastomosis, Anastomotic wound healing