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World J Gastroenterol. Feb 21, 2006; 12(7): 1120-1124 Published online Feb 21, 2006. doi: 10.3748/wjg.v12.i7.1120
ABH and Lewis antigen distributions in blood, saliva and gastric mucosa and H pylori infection in gastric ulcer patients
Luisa Caricio Martins, Tereza Cristina de Oliveira Corvelo, Henrique Takeshi Oti, Rosane do Socorro Pompeu Loiola, Délia Cristina Figueira Aguiar, Katarine Antônia dos Santos Barile, Renata Kelly Costa do Amaral, Hivana Patricia Melo Barbosa, Amanda Alves Fecury, Juciclayton Tavares de Souza
Luisa Caricio Martins, Tereza Cristina de Oliveira Corvelo, Rosane do Socorro Pompeu Loiola, Délia Cristina Figueira Aguiar, Katarine Antônia dos Santos Barile, Renata Kelly Costa do Amaral, Hivana Patricia Melo Barbosa, Amanda Alves Fecury, Juciclayton Tavares de Souza, Immunogenetics Laboratory , Department of Genetics, Federal University of Pará, Brazil
Co-first-authors: Luisa Caricio Martins, Tereza Cristina de Oliveira Corvelo
Co-correspondents: Tereza Cristina de Oliveira Corvelo
Supported by the Secretaria Executiva de Ciência, Tecnologia e Meio Ambiente - SECTAM and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES.
Received: May 20, 2005 Revised: July 2, 2005 Accepted: July 28, 2005 Published online: February 21, 2006
Abstract
AIM: To investigate the ABH and Lewis antigen expression in erythrocytes, saliva and gastric epithelium, as well as the association between H pylori and the presence of gastric epithelial lesions.
METHODS: The distribution of ABH and Lewis blood group antigens in erythrocytes, saliva and gastric mucosa of H pylori-infected gastric ulcer patients was analyzed. Forty-two patients with gastric ulcer were studied, and fifty healthy individuals were used as control group. The blood group antigens were determined by direct hemagglutination, dot-ELISA and immunohistochemical methods in erythrocytes, saliva and gastric mucosa specimens, respectively. Diagnosis for H pylori infection was performed by conventional optical microscopy and ELISA.
RESULTS: A higher seroprevalence of IgG H pylori specific antibodies was observed in gastric ulcer patients (90%) compared to the control group (60%). We observed a significant increase of phenotypes O, A2 and Lewis b in H pylori-infected patients. The expression of these antigens had progressive alterations in areas of ulcerous lesions and intestinal metaplasia.
CONCLUSION: ABH and Lewis blood group antigens are a good indicator for cellular alterations in the gastric epithelium.
