Basic Research
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World J Gastroenterol. Feb 21, 2006; 12(7): 1086-1091
Published online Feb 21, 2006. doi: 10.3748/wjg.v12.i7.1086
Reduced antioxidant level and increased oxidative damage in intact liver lobes during ischaemia-reperfusion
László Váli, Gabriella Taba, Klára Szentmihályi, Hedvig Fébel, Tímea Kurucz, Zsolt Pallai, Péter Kupcsulik, Anna Blázovics
László Váli, Anna Blázovics, Department of of Medicine, Semmelweis University, Budapest, Hungary
Gabriella Taba, Klára Szentmihályi, Chemical Research Center, Hungarian Academy of Sciences
Hedvig Fébel, Research Institute for Animal Breeding and Nutrition, Herceghalom, Hungary
Tímea Kurucz, Zsolt Pallai, Diachem Rt., Budapest, Hungary
Péter Kupcsulik, Department of Surgery, Semmelweis University, Budapest, Hungary
Supported by the Ph.D. Programme of the Semmelweis University No 2/1, ETT 002/2003 Project and 1A 005/2004, 1B 047/2004 NKFP Projects
Correspondence to: Dr.Blázovics Anna, Department of Medicine, Semmelweis University, H-1088 Budapest, Szentkirályiu. 46., Hungary. blaz@bel2.sote.hu
Telephone: +36-705-335012
Received: March 22, 2005
Revised: July 2, 2005
Accepted: July 20, 2005
Published online: February 21, 2006
Abstract

AIM: To determine whether increased blood flow of the liver can cause oxidative stress and hepatocyte damage, and to elaborate methods suitable for measuring the antioxidant defence during hepatic surgery on rat model.

METHODS: In nembutal narcosis, the left lateral and the medial lobes of the liver were clipped for 45 min to make the total blood supply flow through the other lobes. Total antioxidant status, glutathione peroxidase and superoxide dysmutase activity, as well as the concentrations of diene conjugates and free sulphydril groups, H-donating ability and reducing power of the liver samples were determined. Chemiluminescent intensity of the liver was also measured. Metal ions (Al, Ca, Cu, Fe, Mg, Mn, Zn) and P and S concentrations of the liver were determined with an inductively coupled plasma optical emission spectrometer and Se content was measured by cathodic stripping voltammetry.

RESULTS: Glutathione peroxidase and superoxide dysmutase activities of the liver decreased significantly in the hyperemia group compared to those observed in the sham operated group. The level of total antioxidant status was also significantly lower in the hyperemia group. H-donating ability, reducing power and free sulphydril group concentration showed the same tendency. A significant correlation (P<0.05) was found between the changes in non-specific antioxidant activities. This pointed to simultaneous activity of the antioxidant defence system. Al, Cu, Mn, Zn, and S were lower in the hyperemia group than in the sham operated group when the levels of Ca, Fe, Mg, Se and P ions were higher during hyperemia.

CONCLUSION: Oxidative stress is one of the main factors for the injury of intact liver lobes during ischaemia-reperfusion.

Keywords: Free radicals, Liver, Oxidative stress, Metal elements, Redox-balance