Esophageal Cancer
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Nov 28, 2006; 12(44): 7104-7112
Published online Nov 28, 2006. doi: 10.3748/wjg.v12.i44.7104
Identification of squamous cell carcinoma associated proteins by proteomics and loss of beta tropomyosin expression in esophageal cancer
Ferdous Rastgar Jazii, Zahra Najafi, Reza Malekzadeh, Thomas P Conrads, Abed Ali Ziaee, Christian Abnet, Mansour Yazdznbod, Ali Asghar Karkhane, Ghasem H Salekdeh
Ferdous Rastgar Jazii, Zahra Najafi, Ali Asghar Karkhane, National Institute of Genetic Engineering and Biotechnology, PO BOX: 14155 6343, Tehran, Iran
Reza Malekzadeh, Digestive Disease Research Center, Shariati Hospital, Medical University, Tehran, Iran
Thomas P Conrads, Laboratory of Proteomics and Analytical Technologies, SAIC -Frederick B, Frederick, MD 21702, United States
Abed Ali Ziaee, Institute of Biochemistry and Biophysics, Tehran University, Tehran, Iran
Mansour Yazdznbod, Department of surgery, Shariati Hospital, Medical University, Tehran, Iran
Christian Abnet, NCI Center for Cancer Research, Cancer Prevention Studies Branch, Bethesda, United States
Ghasem H Salekdeh, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran
Supported by National Institute of Genetic Engineering and Biotechnology, No. 199 and Digestive Disease Research Center, No. 18/81
Correspondence to: Ferdous Rastgar Jazii, National Institute of Genetic Engineering and Biotechnology, PO BOX: 14155 6343, Tehran, Iran.
Telephone: +98-21-44580380 Fax: +98-21-44580399
Received: August 2, 2006
Revised: August 8, 2006
Accepted: August 22, 2006
Published online: November 28, 2006

AIM: To assess the proteome of normal versus tumor tissue in squamous cell carcinoma of the esophagus (SCCE) in Iranian patients and compare our results with former reports by using proteomics.

METHODS: Protein was extracted from normal and tumor tissues. Two dimensional electrophoresis was carried out and spots with differential expression were identified with mass spectrometry. RNA extraction and RT-PCR along with immunodetection were performed.

RESULTS: Fourteen proteins were found whose expression levels differed in tumor compared to normal tissues. Mass spectrometric analysis resulted in the identification of β-tropomyosin (TMβ), myosin light chain 2 (and its isoform), myosin regulatory light chain 2, peroxyredoxin 2, annexinIand an unknown polypeptide as the down regulated polypeptides in tumor tissue. Heat shock protein 70 (HSP70), TPM4-ALK fusion oncoprotein 2, myosin light polypeptide 6, keratinI, GH16431p and calreticulin were the up-regulated polypeptides found in tumor tissue. Several of these proteins, such as TMβ, HSP70, annexinI, calreticulin, TPM4-ALK and isoforms of myosins, have been well recognized in tumorigenesis of esophageal or other types of cancers.

CONCLUSION: Our study not only supports the involve-ment of some of the formerly reported proteins in SCCE but also introduces additional proteins found to be lost in SCCE, including TMβ.

Keywords: Squamous Cell Carcinoma, Esophagus, Esophageal, Proteomics, Two dimensional electrophore-sis, Polypeptide marker