Identification of differentially expressed genes in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis

doi:10.3748/wjg.v12.i42.6893

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Nov 14, 2006; 12(42): 6893-6897
Published online Nov 14, 2006. doi: 10.3748/wjg.v12.i42.6893

Identification of differentially expressed genes in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis

Xiao-Nan Cui, Jian-Wu Tang, Li Hou, Bo Song, Li-Ying Ban

Xiao-Nan Cui, Li-Ying Ban, Department of Oncology, The 1st Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China

Jian-Wu Tang, Li Hou, Bo Song, Department of Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China

Author contributions: All authors contributed equally to the work.

Supported by National Natural Science Foundation of China, No. 30500586

Correspondence to: Jian-Wu Tang, Department of Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China. jianwutang@163.net

Telephone: +86-411-84720021

Received: July 24, 2006
Revised: September 6, 2006
Accepted: September 12, 2006
Published online: November 14, 2006

Abstract

AIM: To identify genes differentially expressed in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis.

METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with low potential of lympho-genous metastasis Hca-P and its synogenetic cell line Hca-F with high metastatic potential was constructed by suppression subtracted hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed.

RESULTS: Fifteen differentially expressed cDNA fragments of Hca-P were obtained which revealed 8 known genes, 4 expressed sequence tags (ESTs) and 3 cDNAs showed no homology.

CONCLUSION: Tumor metastasis is an incident involving multiple genes. SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.

Keywords: Suppression subtracted hybridization, Liver neoplasm, Metastasis suppression genes