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World J Gastroenterol. Sep 21, 2006; 12(35): 5705-5710
Published online Sep 21, 2006. doi: 10.3748/wjg.v12.i35.5705
Assessment of oxidative stress in chronic pancreatitis patients
Mariette Verlaan, Hennie MJ Roelofs, Annie van Schaik, Geert JA Wanten, Jan BMJ Jansen, Wilbert HM Peters, Joost PH Drenth
Mariette Verlaan, Hennie MJ Roelofs, Annie van Schaik, Geert JA Wanten, Jan BMJ Jansen, Wilbert HM Peters, Joost PH Drenth, Department of Gastroenterology, Radboud University Nijmegen Medical Centre, The Netherlands
Author contributions: All authors contributed equally to the work.
Supported by a grant from the Dutch Foundation of Digestive Diseases
Correspondence to: Mariette Verlaan, Department of Gastroenterology, Radboud University Nijmegen Medical Centre, PO Box 9101, Nijmegen 6500 HB, The Netherlands. m.verlaan@mdl.umcn.nl
Telephone: +31-24-3616520 Fax: +31-24-3540103
Received: May 16, 2005
Revised: July 15, 2005
Accepted: July 28, 2005
Published online: September 21, 2006
Abstract

AIM: To assess the levels of antioxidant capacity and oxidative damage in blood of chronic pancreatitis (CP) patients in comparison with those in healthy control subjects, by using several different analytical techniques.

METHODS: Thirty-five CP patients and 35 healthy control subjects were investigated prospectively with respect to plasma levels of thiols, ferric reducing ability of plasma (FRAP, i.e. antioxidant capacity), levels of protein carbonyls and thiobarbituric acid reactive substances (TBARS). Additionally, we evaluated the production of reactive oxygen species (ROS) in whole blood.

RESULTS: The antioxidative thiols including cysteine, cysteinylglycine and glutathione were significantly lower in CP patients. In addition, the non-enzymatic antioxidant capacity was significantly lower in CP patients, which correlated with the amount of oxidative protein (protein carbonyls) and the extent of lipid damage (TBARS), both were significantly higher in CP patients. The ROS production in whole blood after stimulation with phorbol 12-myritate 13-acetaat, demonstrated a strong tendency to produce more ROS in CP patients.

CONCLUSION: Oxidative stress may contribute to the pathogenesis of chronic pancreatitis by decreasing antioxidant capacity and increasing oxidative damage in CP patients may be a rationale for intervention with antioxidant therapy.

Keywords: Chronic pancreatitis, Oxidative stress, Thiols, Ferric reducing ability of plasma, Protein carbonyls, Thiobarbituric acid reactive substances, Reactive oxygen species