Basic Research
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Sep 7, 2006; 12(33): 5326-5330
Published online Sep 7, 2006. doi: 10.3748/wjg.v12.i33.5326
Evaluation of IGL-1 preservation solution using an orthotopic liver transplantation model
Hassen Ben Abdennebi, Ziad Elrassi, Jean-Yves Scoazec, Jean-Paul Steghens, Silvina Ramella-Virieux, Olivier Boillot
Hassen Ben Abdennebi, Department of Humane Physiology, Faculty of Pharmacy, Monastir 5000, Tunisia
Ziad Elrassi, Jean-Yves Scoazec, Olivier Boillot, Hepatic Transplantation Unit, Edouard Herriot Hospital, Lyon 69437, France
Jean-Paul Steghens, Department of Biochemistry, Edouard Herriot Hospital, Lyon 69437, France
Silvina Ramella-Virieux, Georges Lopez Institute, Civrieux d’Azergues 69380, France
Correspondence to: Hassen Ben Abdennebi, 1 rue Avicenne, Department of Humane Physiology, Faculty of Pharmacy, Monastir 5000, Tunisia.
Telephone: +216-7-3461000
Received: March 28, 2006
Revised: March 28, 2006
Accepted: April 24, 2006
Published online: September 7, 2006

AIM: To compare, in a pig liver transplantation model, the protective effect of UW with that of IGL-1, a high-sodium preservation solution containing polyethylene glycol (PEG) as an oncotic supply.

METHODS: All livers were harvested and grafted orthotopically according to standard techniques. The livers were washed out and preserved for 7 h in IGL-1 (n = 6) or in UW solution (n = 7) at 4°C. In a sham group (n = 4), the livers underwent a 60-min warm ischemia at 37°C. The hepatocellular injury was assessed in organ preservation solution washed out from the graft at the end of ischemic storage (before revascularization), and in serum 2 h after reperfusion and daily for up to 6 d.

RESULTS: Livers preserved in IGL-1 solution released markedly less AST than that preserved in the UW solution before and after revascularization (P < 0.05). Besides, the activity of creatine kinase-BB, a marker of sinusoidal lining cells injury, was higher in the UW group than in the IGL-1 group (P < 0.05). Histological results showed less necrotic regions in livers preserved in IGL-1 solution; however, no difference was observed for inflammation.

CONCLUSION: IGL-1 liquid effectively protects parenchymal and non-parenchymal cells against prese-rvation-reperfusion injuries.

Keywords: University of Wisconsin solution, IGL-1 liquid, Cold preservation, Liver transplantation, Sinusoidal endothelial cells