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World J Gastroenterol. Aug 7, 2006; 12(29): 4745-4749
Published online Aug 7, 2006. doi: 10.3748/wjg.v12.i29.4745
Role of detection of microsatellite instability in Chinese with hereditary nonpolyposis colorectal cancer or ordinary hereditary colorectal cancer
Wen-Zhi Liu, Feng Jin, Zhen-Hai Zhang, Shu-Bao Wang
Wen-Zhi Liu, Feng Jin, Shu-Bao Wang, Department of Oncology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Zhen-Hai Zhang, The Second Department of General Surgery, Second Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, China
Correspondence to: Feng Jin, Department of Oncology, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. lwz65888@yahoo.com.cn
Telephone: +86-024-23281706
Received: March 14, 2006
Revised: March 28, 2006
Accepted: April 21, 2006
Published online: August 7, 2006
Abstract

AIM: To detect microsatellite instability (MSI) in patients with hereditary nonpolyposis colorectal cancer or ordinary hereditary colorectal cancer and to provide criteria for screening the kindreds with hereditary nonpolyposis colorectal cancer at molecular level.

METHODS: MSI was detected in the specimens from 20 cases with HNPCC, 20 cases with ordinary hereditary colorectal cancer and 20 cases with sporadic colorectal cancer by means of polymerase chain reaction-single strand conformation polymorphism.

RESULTS: The positive rate of MSI was 85% (17/20) in HNPCC group, 40% (8/20) in ordinary hereditary colorectal cancer group and 10% (2/20) in the sporadic colorectal cancer group respectively. The differences were significant. The mean ages of the three groups were 43.6, 52.2, and 61.8 years respectively, which increased gradually. The incidence of right hemicolon cancer was 64.7%, 37.5%, and 0% respectively, which decreased gradually and had significant difference. The expression ratio of BAT26 and BAT25 was 94.1% respectively, which was highest in the 5 gene sites studied. The incidence of poorly differentiated adenocarcinoma was 70.6% in HNPCC group among high frequency microsatellite instability (MSI-H), which was higher than the other two groups, which had 50% and 50% respectively.

CONCLUSION: The incidence of MSI-H is higher in HNPCC group. The detection of MSI is simple and economical and has high correlation with the clinicopathologic feature of HNPCC and can be used as a screening method to detect the germ line mutation of the mismatch repair gene.

Keywords: Hereditary nonpolyposis colorectal cancer, Microsatellite instability, Ordinary hereditary colorectal cancer, Single strand conformation polymorphism, Polymerase chain reaction