Changes of inducible protein-10 and regulated upon activation, normal T cell expressed and secreted protein in acute rejection of pancreas transplantation in rats

doi:10.3748/wjg.v12.i26.4156

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jul 14, 2006; 12(26): 4156-4160
Published online Jul 14, 2006. doi: 10.3748/wjg.v12.i26.4156

Changes of inducible protein-10 and regulated upon activation, normal T cell expressed and secreted protein in acute rejection of pancreas transplantation in rats

Jun Zhu, Ze-Kuan Xu, Yi Miao, Xun-Liang Liu, Hong Zhang

Jun Zhu, Ze-Kuan Xu, Yi Miao, Xun-Liang Liu, Department of General Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China

Jun Zhu, Hong Zhang, Department of Experimental Surgery, Xuzhou Medical College, Xuzhou 221004, Jiangsu Province, China

Author contributions: All authors contributed equally to the work.

Supported by a grant from the “135” Foundation of Jiangsu Province, No. 2003-19

Correspondence to: Dr. Ze-Kuan Xu, Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. xuzekuan@hotmail.com

Telephone: +86-25-83718836

Received: April 6, 2006
Revised: April 15, 2006
Accepted: April 24, 2006
Published online: July 14, 2006

Abstract

AIM: To investigate the role of IFN-γ inducible protein -10 (IP-10) and regulated upon activation, normal T cell expressed and secreted (RANTES) protein in acute pancreatic allograft rejection in rats.

METHODS: An experimental pancreas transplantation model was established using diabetic SD rats as the recipient, induced by applying streptozocin (STZ). Pancreas transplantation was performed with a physiologic method of portal venous and enteric drainage. Rats were divided into two groups, isograft group (group A, n = 24) and allograft group (group B, n = 24) in which either healthy SD rats or Wistar rats served as donors, respectively. Twelve diabetic or healthy SD rats were used as controls. At d 1, 4, 7, and 10 post transplantation, serum IP-10 and RANTES were assessed by ELISA and their expression in the allografts was determined by immunohistochemistry.

RESULTS: In group B (allograft group), the development of acute rejection was significantly correlated with increased serum concentration and tissue expression of IP-10 and RANTES, with a peak level at d 7 post transplantation. In contrast, there was no obvious change before and after transplantation in group A (isograft group).

CONCLUSION: Our study suggests a possible role of IP-10 and RANTES in acute rejection and early monitoring of chemokines may be helpful in predicting the outcome of pancreas transplantation.

Keywords: Pancreas transplantation, Chemokine, Rats