Colorectal Cancer
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Jul 7, 2006; 12(25): 3983-3988
Published online Jul 7, 2006. doi: 10.3748/wjg.v12.i25.3983
Effects of adenoviral-mediated gene transduction of NK4 on proliferation, movement, and invasion of human colonic LS174T cancer cells in vitro
Jian-Zheng Jie, Jian-Wei Wang, Jian-Guo Qu, Wei Wang, Tao Hung
Jian-Zheng Jie, Jian-Wei Wang, Jian-Guo Qu, Tao Hung, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
Jian-Zheng Jie, Wei Wang, Department of General Surgery, China-Japan Union Hospital, Jilin University, Changchun 130031, Jilin Province, China
Co-first author: Jian-Wei Wang
Correspondence to: Dr. Jian-Wei Wang, State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Chinese Academy of Medical Sciences, 9 # Dong Dan San Tiao, Dongcheng District, Beijing 100730, China. wangjw28@vip.sina.com
Telephone: +86-10-65105188 Fax: +86-10-65105188
Received: January 18, 2006
Revised: January 20, 2006
Accepted: January 24, 2006
Published online: July 7, 2006
Abstract

AIM: To investigate the inhibitory effects of a recombinant adenovirus vector that expresses NK4, a truncated form of human hepatocyte growth factor (HGF), on human colonic adenocarcinoma cells in vitro to establish a basis for future NK4 gene cancer therapy.

METHODS: Cells from the LS174T human colonic adenocarcinoma cell line were infected with recombinant adenovirus rvAdCMV/NK4 and the effects of the manipulation on tumor cell proliferation, scatter, migration, and basement membrane invasion were assessed. Cells infected with a recombinant adenovirus vector (Ad-LacZ) expressing β-galactosidase served as the controls.

RESULTS: We found that rvAdCMV/NK4 expression attenuated HGF-induced tumor cell scatter, migration, and basement membrane invasion (P < 0.05), but did not inhibit tumor cell proliferation.

CONCLUSION: HGF-induced LS174T tumor cell scatter, migration, and invasion can be antagonized by the recombinant NK4-expressing adenovirus.

Keywords: Human colonic adenocarcinoma; NK4; Hepatocyte growth factor; Adenoviral vector