Clinical Research
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Jun 14, 2006; 12(22): 3593-3596
Published online Jun 14, 2006. doi: 10.3748/wjg.v12.i22.3593
Effect of abdominal trauma on hemorrhagic shock-induced acute lung injury in rats
Bulent Kilicoglu, Erol Eroglu, Sibel Serin Kilicoglu, Kemal Kismet, Fusun Eroglu
Bulent Kilicoglu, Kemal Kismet, Department of Surgery, Ankara Training and Research Hospital, Ulucanlar, Ankara, Turkey
Erol Eroglu, Department of Surgery, Suleyman Demirel University School of Medicine, Isparta, Turkey
Sibel Serin Kilicoglu, Department of Histology and Embryology, Ankara University School of Medicine, Ankara, Turkey
Fusun Eroglu, Department of Anesthesiology and Reanimation, Suleyman Demirel University School of Medicine, Isparta, Turkey
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Bulent Kilicoglu, Ankara Egitim ve Arastirma Hastanesi 4th, Genel Cerrahi Klinigi, 06340, Ankara, Turkey. bulentkilicoglu@yahoo.com.tr
Telephone: +90-312-5953449 Fax: +90-312-3633396
Received: February 8, 2006
Revised: February 18, 2006
Accepted: February 28, 2006
Published online: June 14, 2006
Abstract

AIM: To evaluate the effects of abdominal trauma on hemorrhagic shock-induced acute lung injury in rats.

METHODS: Five groups were allocated (n = 8) in the study. Group I was taken as the control group, group II as the hemorrhagic shock group, group III as hemorrhagic shock + laparotomy, group IV as hemorrhagic shock + splenectomy and group V as splenectomy + omentectomy + hemorrhagic shock group. Hemorrhagic shock was induced by drawing blood and reducing mean arterial pressure (MAP) to 40 mmHg within 10 min. After a hypotensive period of 1 h, animals were resuscitated. Bronchoalveolar lavage (BAL) was performed to recover cells from the alveolar space with 40 mL of BAL fluid after resuscitation malondialdehyde (MDA) and L-γ-glutamyl-L-cysteinyl-glycine (GSH) levels were measured in serum, erythrocytes and lung tissue.

RESULTS: Serum, erythrocyte, lung tissue MDA and GSH levels were significantly increased in hemorrhagic shock groups II-V (P < 0.05). Lymphocyte, neutrophil and alveolar macrophage counts in BAL fluid indicated a significant difference between control and shock groups (P < 0.05).

CONCLUSION: The degree of trauma increases hemorrhagic shock-induced acute lung injury.

Keywords: Hemorrhagic shock; Acute lung injury; Abdominal trauma