Basic Research
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World J Gastroenterol. Apr 28, 2006; 12(16): 2536-2548
Published online Apr 28, 2006. doi: 10.3748/wjg.v12.i16.2536
A comparison of gene expression in mouse liver and kidney in obstructive cholestasis utilizing high-density oligonucleotide microarray technology
Gerald U Denk, Shi-Ying Cai, Wen-Sheng Chen, Aiping Lin, Carol J Soroka, James L Boyer
Gerald U Denk, Shi-Ying Cai, Wen-Sheng Chen, Carol J Soroka, James L Boyer, Liver Center, Yale University School of Medicine, New Haven, Connecticut, United States
Aiping Lin, W. M. Keck Biotechnology Resource Laboratory, Yale University School of Medicine, New Haven, Connecticut, United States
Gerald U Denk, Department of Medicine II-Großhadern, Ludwig-Maximilians University, München, Germany
Wen-Sheng Chen, Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
Co-first-author: Gerald U Denk and Shi-Ying Cai
Supported by the USPHS grants DK 25636 (J. L. B.), the Yale Liver Center Cellular and Molecular Physiology and Morphology Cores (P30-34989), and the Deutsche Forschungsgemeinschaft Grant DE 872/1-1 (G. U. D.)
Correspondence to: James L Boyer, MD, Ensign Professor of Medicine, Director, Liver Center, Yale University School of Medicine, PO Box 208019, 333 Cedar Street, 1080 LMP, New Haven, Connecticut 06520-8019, United States.
Telephone: +1-203-7855279 Fax: +1-203-7857273
Received: June 29, 2005
Revised: December 1, 2005
Accepted: December 7, 2005
Published online: April 28, 2006

AIM: To assess the effects of obstructive cholestasis on a wider range of gene expression using microarray technology.

METHODS: Male C57BL/6J mice underwent common bile duct ligation (BDL) and were matched with pair-fed sham-operated controls. After 7 d, the animals were sacrificed and total RNA was isolated from livers and kidneys. Equal amounts of RNA from each tissue were pooled for each group and hybridized to Affymetrix GeneChip®MG-U74Av2 containing a total of 12 488 probe sets. Data analysis was performed using GeneSpring®6.0 software. Northern analysis and immunofluorescence were used for validation.

RESULTS: In sham-operated and BDL mice, 44 and 50% of 12 488 genes were expressed in livers, whereas 49 and 51% were expressed in kidneys, respectively. Seven days after BDL, 265 liver and 112 kidney genes with GeneOntology annotation were up-regulated and 113 liver and 36 kidney genes were down-regulated in comparison with sham-operated controls. Many genes were commonly regulated in both tissues and metabolism-related genes represented the largest functional group.

CONCLUSION: Following BDL, microarray analysis reveals a broad range of gene alterations in both liver and kidney.

Keywords: Bile duct ligation, Cholestasis, Kidney, Liver, Microarray