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World J Gastroenterol. Apr 21, 2006; 12(15): 2437-2440
Published online Apr 21, 2006. doi: 10.3748/wjg.v12.i15.2437
Identification of a new target region on the long arm of chromosome 7 in gastric carcinoma by loss of heterozygosity
De-Sheng Weng, Jin-Tian Li, Shi-Juan Mai, Zhi-Zhong Pan, Bing-Jian Feng, Qi-Sheng Feng, Li-Xi Huang, Qi-Jing Wang, Yong-Qiang Li, Xing-Juan Yu, Shi-Ping Chen, Jia He, Jian-Chuan Xia
De-Sheng Weng, Jin-Tian Li, Shi-Juan Mai, Zhi-Zhong Pan, Bing-Jian Feng, Qi-Sheng Feng, Li-Xi Huang, Qi-Jing Wang, Yong-Qiang Li, Xing-Juan Yu, Shi-Ping Chen, Jia He, Jian-Chuan Xia, Research Section of Oncopathology, State Key Laboratory of Oncology in Southern China, Cancer Center, Zhongshan University, Guangzhou 510064, Guangdong Province, China
Supported by the National Natural Science Foundation of China, No. 30471950 and the Key Project of Natural Science Foundation of Guangdong Province, No. 04105350
Correspondence to: Professor Jian-Chuan Xia, Research Section of Oncology, State Key Laboratory of Oncology in Southern China, Cancer Center, Zhongshan University, Guangzhou 510060, Guangdong Province, China. xiajc51@hotmail.com
Telephone: +86-20- 87343173 Fax: +86-20-87343173
Received: August 31, 2005
Revised: October 2, 2005
Accepted: October 10, 2005
Published online: April 21, 2006
Abstract

AIM: To define the common deleted region on the long arm of haman chromosome 7q linked to primary gastric carcinomas in Chinese by loss of heterozygosity (LOH) and its clinical significance.

METHODS: Nine microsatellite markers distributed over chromosome 7q with an average marker density of 10cM were used to examine 70 primary gastric carcinomas for LOH by PCR amplification. The PCR products were separated by electrophoresis on polyacrylamide gel. Genescan and Genotyper softwares were used to analyze LOH.

RESULTS: LOH with at least one marker on 7q occurred in 34.3% (12/50) of the tumors. Among them, LOH at D7S486 and D7S798 was higher in 24.0% (24/70) and 19.2% (5/26), respectively. By statistical analysis we also observed an obvious genotype-phenotype correlation on 7q (P < 0.05). The frequency of LOH at D7S486 in patients with lymph node metastasis was significantly higher than that in those without lymph node metastasis (P = 0.015).

CONCLUSION: The high incidence of LOH at D7S486 and its correlation with poorer prognosis suggest that there might be putative tumor suppressor genes in this region involved in the tumorigenesis and progression of gastric carcinoma.

Keywords: LOH, Chromosome 7, Gastric cancer